Abstract | BACKGROUND: STUDY DESIGN AND METHODS: A total of 8171 volunteer blood donors were prospectively recruited by six US blood centers from December 2006 to May 2007. Donors provided a detailed history of pregnancy and transfusion and a sample for HLA Class I and II antibody testing by multiantigen bead flow analysis. RESULTS: A total of 8171 donors were enrolled; 7920 (96.9%) had valid HLA antibody test results and 7841 (99%) of those had complete pregnancy and transfusion information. The prevalence of any HLA antibody was similar in nontransfused (n = 1138) and transfused (n = 895) men, 1.0% versus 1.7% (p = 0.16). HLA antibodies were detected in 17.3% of all female donors (n = 5834) and in 24.4% of those with a history of previous pregnancy (n = 3992). The prevalence of HLA antibodies increased in women with greater numbers of pregnancy: 1.7% (zero), 11.2% (one), 22.5% (two), 27.5% (three), and 32.2% (four or more pregnancies; p < 0.0001). CONCLUSION: HLA Class I and Class II antibodies are detectable at low prevalence in male donors regardless of transfusion and in female donors without known immunizing events. The prevalence of HLA antibodies increases significantly with more pregnancies. These data will allow blood centers to estimate the impact of HLA antibody testing as a potential TRALI risk reduction measure.
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Authors | Darrell J Triulzi, Steven Kleinman, Ram M Kakaiya, Michael P Busch, Philip J Norris, Whitney R Steele, Simone A Glynn, Christopher D Hillyer, Patricia Carey, Jerome L Gottschall, Edward L Murphy, Jorge A Rios, Paul M Ness, David J Wright, Danielle Carrick, George B Schreiber |
Journal | Transfusion
(Transfusion)
Vol. 49
Issue 9
Pg. 1825-35
(Sep 2009)
ISSN: 1537-2995 [Electronic] United States |
PMID | 19453983
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Chemical References |
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Topics |
- Acute Lung Injury
(etiology, immunology, prevention & control)
- Adult
- Aged
- Antibodies
(blood, immunology)
- Blood Donors
(statistics & numerical data)
- Blood Transfusion
(statistics & numerical data)
- Female
- HLA Antigens
(immunology)
- Humans
- Male
- Middle Aged
- Pregnancy
- Sex Factors
- Transfusion Reaction
- Young Adult
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