Ectopic expression of a rice protein phosphatase 2C gene OsBIPP2C2 in tobacco improves disease resistance.

Abstract

Protein phosphatase 2Cs (PP2Cs) have been demonstrated to play critical roles in regulation of plant growth/development, abscisic acid signaling pathway and adaptation to environmental stresses. Here we report the cloning and molecular characterization of a novel rice protein phosphatase 2C gene, OsBIPP2C2 (Oryza sativa L. BTH-induced protein phosphatase 2C 2). OsBIPP2C2 has three alternatively spliced transcripts and the largest transcript OsBIPP2C2a encodes a 380 aa protein containing all 11 conserved catalytic subdomains of PP2Cs. Expression of OsBIPP2C2a was significantly induced by benzothiadiazole (BTH), one of defense-related signal molecules in plants. Expression of OsBIP2C2a was induced by infection with the blast fungus, Magnaporthe grisea, and the pathogen-induced expression of OsBIPP2C2a in BTH-treated rice seedlings was much earlier and stronger than those in water-treated seedlings. Overexpression of OsBIPP2C2a in transgenic tobacco plants resulted in increased disease resistance against tobacco mosaic virus and Phytophthora parasitica var. nicotianae. Importantly, the OsBIPP2C2a-overexpressing transgenic tobacco plants showed constitutive expression of defense-related genes. These results suggest that OsBIPP2C2a may play an important role in disease resistance through activation of defense response.

• Authors: Xuebo Hu, Huijuan Zhang, Guojun Li, Yuxia Yang, Zhong Zheng, Fengming Song
• Journal: Plant cell reports (Plant Cell Rep) Vol. 28 Issue 6 Pg. 985-95 (Jun 2009) ISSN: 1432-203X [Electronic] Germany
• PMID: 19381642 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Plant
• Plant Proteins
• Thiadiazoles
• benzo-1,2,3-thiadiazole
• Phosphoprotein Phosphatases
• Protein Phosphatase 2C

Topics

• Alternative Splicing
• Amino Acid Sequence
• Cloning, Molecular
• DNA, Plant (genetics)
• Gene Expression Regulation, Plant
• Genes, Plant
• Immunity, Innate
• Magnaporthe (physiology)
• Molecular Sequence Data
• Oryza (genetics)
• Phosphoprotein Phosphatases (genetics, metabolism)
• Plant Diseases (genetics)
• Plant Proteins (genetics, metabolism)
• Plants, Genetically Modified (genetics, immunology)
• Protein Phosphatase 2C
• Sequence Alignment
• Sequence Analysis, DNA
• Thiadiazoles (pharmacology)
• Tobacco (genetics, immunology)