Lesion evolution during focal
cerebral ischemia may depend on flow restrictions or on accumulation of toxic mediators within the
infarct and expansion of these factors to the periinfarct region. So far, the precise contribution of flow dependent versus spreading-mediated impairment of viable periinfarct tissue has not been determined. Therefore, we measured lesion expansion, flow restrictions and
glutamate distribution on serial brain sections at different time points after experimental focal
ischemia. Permanent focal
ischemia was induced by occlusion of the right middle cerebral artery in male rats and the flow reduction was subsequently measured at 1, 12 and 24 h using iodo[14C]
antipyrine autoradiography. Additionally, the necrotic volume was determined on serial brain sections and the
glutamate content was measured in tissue samples from adjacent microdissections. Twelve hours after focal
ischemia no noteworthy viable areas with blood flow restrictions of 20-40 ml 100 g(-1) min(-1) existed but at 24 h the necrotic tissue exceeded the hemodynamically compromised region by 40 +/- 21 mm3 (24%). Furthermore, at 12 and 24 h the
glutamate content was elevated in areas surrounding the
infarct. Relevant flow restrictions are detectable only during early stages of
infarct maturation, whereas the propagation of secondary factors may be the predominant mechanism for delayed
infarct evolution.