Conditioning vaccination site with irradiated MIP-3alpha-transfected tumor cells enhances efficacy of dendritic cell-based cancer vaccine.

Abstract

Macrophage inflammation protein-3alpha (MIP-3alpha) is a chemokine expressed in inflamed tissue and capable of inducing migration of immature dendritic cells (DCs) or Langerhans cells. We postulated that conditioning vaccination sites with MIP-3alpha might enhance the efficacy of subsequently administered DC-based cancer vaccines. Our results demonstrate that subcutaneously injection of irradiated tumor cells expressing MIP-3alpha induces substantial cell infiltration to the injection site. Vaccination of irradiated tumor cells expressing MIP-3alpha followed by DCs pulsed with irradiated tumor cells can effectively suppress tumor growth in animals, which is significantly better than vaccination with irradiated MIP-3alpha-producing tumor cells or DCs pulsed with tumor cells alone. The protective effect was most evident when the MIP-3alpha-producing tumor cells and DC-based vaccines were injected at the same site. These results support the notion that this combination vaccination strategy might generate a more effective immune response to suppress the growth of tumor cells in animals.

• Authors: Neng-Yao Shih, Hui-Yu Yang, Hui-Ting Cheng, Yi-Mei Hung, Yi-Chuan Yao, Yun-Han Zhu, Yu-Chen Wu, Ko-Jiunn Liu
• Journal: Journal of immunotherapy (Hagerstown, Md. : 1997) (J Immunother) Vol. 32 Issue 4 Pg. 363-9 (May 2009) ISSN: 1537-4513 [Electronic] United States
• PMID: 19342969 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cancer Vaccines
• Chemokine CCL20

Topics

• Animals
• Cancer Vaccines (administration & dosage, immunology)
• Cell Line, Tumor
• Cell Movement
• Chemokine CCL20 (immunology)
• Dendritic Cells (immunology, transplantation)
• Lung Neoplasms (prevention & control, secondary)
• Melanoma, Experimental (prevention & control, secondary, therapy)
• Mice
• Mice, Inbred C57BL
• Transfection