Association of metastin/a G-protein-coupled receptor signaling and Down syndrome critical region 1 in epithelial ovarian cancer.

Abstract

UNLABELLED:

It has been revealed that metastin/a G-protein-coupled receptor (AXOR12) signaling enhances the expression of Down syndrome critical region 1 (DSCR1), known to be duplicated in Down syndrome, and suppresses tumor metastasis in in vitro study. The aim of this study was to evaluate whether gene expression of metastin/AXOR12 signaling system is correlated with that of DSCR1 and consequently affect prognosis of patients with epithelial ovarian cancer.

PATIENTS AND METHODS:

The expression levels of metastin, AXOR12, DSCR1 isoform 1 (DSCR1-1), DSCR1 isoform 4 (DSCR1-4), calcineurin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene expression were analyzed by real-time quantitative reverse transcription-polymerase chain reaction in 102 epithelial ovarian cancer surgical specimens.

RESULTS:

Patients were dichotomized into two groups having low and high expressions by using the median value as the cut-off A good agreement was noticed between metastin and AXOR12 gene expression levels (kappa coefficient; 0.73), however, the gene expression of metastin/AXOR12 signaling system was not significantly correlated with that of DSCR1-4. By univariate Cox regression analysis, the prognosis of the patients with low metastin and low AXOR12 gene expression was significantly worse than that of those with high metastin and high AXOR12 gene expression, respectively (p = 0.04 and 0.018). Combination of metastin and AXOR12 gene expression also had significant impact on patient prognosis (p = 0.045). The DSCR1-1, DSCR1-4 and calcineurin gene expressions did not significantly affect the prognosis.

CONCLUSION:

The precise mechanism of metastin/ AXOR12 signaling for suppression of the invasive phenotype in vivo, especially in epithelial ovarian cancer, is still uncertain. Genes such as DSCR1 that are duplicated in Down syndrome might not play an important role in tumorigenesis of epithelial ovarian cancer.

• Authors: Kohkichi Hata, Yoh Watanabe, Hidekatsu Nakai, Takashi Minami, Hiroyuki Ohsaki, Eiichiro Hirakawa, Hiroshi Hoshiai
• Journal: Anticancer research (Anticancer Res) Vol. 29 Issue 2 Pg. 617-23 (Feb 2009) ISSN: 0250-7005 [Print] Greece
• PMID: 19331211 (Publication Type: Journal Article)

Chemical References

• DNA-Binding Proteins
• Intracellular Signaling Peptides and Proteins
• KISS1R protein, human
• Muscle Proteins
• RCAN1 protein, human
• Receptors, G-Protein-Coupled
• Receptors, Kisspeptin-1

Topics

• Adult
• Aged
• Aged, 80 and over
• DNA-Binding Proteins
• Female
• Gene Expression
• Humans
• Immunohistochemistry
• Intracellular Signaling Peptides and Proteins (genetics, metabolism)
• Middle Aged
• Muscle Proteins (biosynthesis, genetics, metabolism)
• Ovarian Neoplasms (genetics, metabolism, pathology)
• Receptors, G-Protein-Coupled (biosynthesis, genetics, metabolism)
• Receptors, Kisspeptin-1
• Signal Transduction