[Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides].

Abstract

OBJECTIVE:

To screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.

METHODS:

Serum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.

RESULTS:

In all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.

CONCLUSION:

SELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.

• Authors: Jie Liu, Yan-Hua Zheng, Jian-Zhong Zhang, Bao-Xi Wang
• Journal: Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae (Zhongguo Yi Xue Ke Xue Yuan Xue Bao) Vol. 31 Issue 1 Pg. 13-6 (Feb 2009) ISSN: 1000-503X [Print] China
• PMID: 19317049 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Blood Proteins

Topics

• Biomarkers, Tumor (blood)
• Blood Proteins (analysis)
• Humans
• Mycosis Fungoides (blood)
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)