15-Epi-lipoxin A(4) inhibits the progression of endometriosis in a murine model.

Abstract

OBJECTIVE:

To examine the pro-resolution actions of 15-epi-lipoxin A(4) (LXA(4)) on endometriotic lesions, on the concentrations and activities of matrix metalloproteinases (MMP-2 and MMP-9) in murine endometriosis.

DESIGN:

Prospective, vehicle-controlled experimental study.

SETTING:

Animal research facility.

ANIMAL(S):

BALB/c mice.

INTERVENTION(S):

Endometriosis (EM) was induced in 30 mice. Fifteen of them were administered LXA(4) for 24 days (LXA(4) group), whereas the other 15 served as a control group (EM group). Another 15 sham-operated mice (sham-operated group) were treated with vehicles.

MAIN OUTCOME MEASURE(S):

The weight of the endometriotic lesions was measured. The concentrations, mRNA, and activities of MMP-2 and MMP-9 were determined by enzyme-linked immunosorbent assay, quantitative real-time polymerase chain reaction, and gelatin zymography, respectively.

RESULT(S):

Compared with EM group, the weight of the endometriotic lesions was decreased, the concentrations of MMP-2 and MMP-9 dropped, the mRNA levels of MMP-2 and MMP-9 in the peritoneal fluid cells and the endometriotic lesions were reduced, and the activities of MMP-2 and MMP-9 were inhibited in the LXA(4) group.

CONCLUSION(S):

LXA(4) may inhibit the progression of endometriosis possibly by lowering the concentrations and the activities of MMP-2 and MMP-9.

• Authors: Qiong-Hua Chen, Wei-Dong Zhou, De-Min Pu, Qian-Sheng Huang, Tian Li, Qing-Xi Chen
• Journal: Fertility and sterility (Fertil Steril) Vol. 93 Issue 5 Pg. 1440-7 (Mar 15 2010) ISSN: 1556-5653 [Electronic] United States
• PMID: 19268934 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2010 American Society for Reproductive Medicine. Published by Elsevier Inc. All rights reserved.

Chemical References

• Anti-Inflammatory Agents, Non-Steroidal
• Lipoxins
• RNA, Messenger
• lipoxin A4
• Matrix Metalloproteinase 2
• Mmp2 protein, mouse
• Matrix Metalloproteinase 9
• Mmp9 protein, mouse

Topics

• Animals
• Anti-Inflammatory Agents, Non-Steroidal (administration & dosage, pharmacology)
• Ascitic Fluid (cytology, enzymology)
• Disease Models, Animal
• Disease Progression
• Down-Regulation
• Endometriosis (drug therapy, enzymology, pathology)
• Endometrium (drug effects, enzymology, pathology, transplantation)
• Enzyme-Linked Immunosorbent Assay
• Estrus
• Female
• Gene Expression Regulation, Enzymologic (drug effects)
• Injections, Intraperitoneal
• Lipoxins (administration & dosage, pharmacology)
• Matrix Metalloproteinase 2 (genetics, metabolism)
• Matrix Metalloproteinase 9 (genetics, metabolism)
• Mice
• Mice, Inbred BALB C
• RNA, Messenger (metabolism)
• Reverse Transcriptase Polymerase Chain Reaction