beta-Glucan administration enhances disease resistance and some innate immune responses in zebrafish (Danio rerio).

Abstract

The present study was conducted to investigate the effect of beta-glucan (derived from Saccharomyces cerevisiae) on the immune response and its protection against an infection of the bacterial pathogen Aeromonas hydrophila in zebrafish (Danio rerio). Zebrafish received beta-glucan by intraperitoneal injection at three different concentrations (5, 2 and 0.5 mgml(-1)) at 6, 4 and 2 days prior the challenge. On challenge day the control and beta-glucan pretreated zebrafish were intraperitoneally injected with A. hydrophila and mortality was recorded for 4 days. Intraperitoneal injection of 5 mgml(-1) of beta-glucan significantly reduced the mortality. A single injection of 5 mgml(-1) of beta-glucan 6 days before challenge also enhanced significantly the survival against the infection. The treatment with beta-glucan increased the myelomonocytic cell population in the kidney at 6h postchallenge with A. hydrophila. Moreover it enhanced the ability of kidney cells to kill A. hydrophila. beta-glucan did not affect the expression of TNFalpha or IL-1 beta but seemed to modulate IFNgamma and chemokine expression in kidney.

• Authors: Iván Rodríguez, Rubén Chamorro, Beatriz Novoa, Antonio Figueras
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 27 Issue 2 Pg. 369-73 (Aug 2009) ISSN: 1095-9947 [Electronic] England
• PMID: 19232393 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Cytokines
• beta-Glucans

Topics

• Adjuvants, Immunologic (pharmacology)
• Aeromonas hydrophila (physiology)
• Animals
• Cytokines (blood)
• Fish Diseases (immunology, mortality)
• Gene Expression Regulation (drug effects)
• Gram-Negative Bacterial Infections (immunology, mortality, veterinary)
• Immunity, Innate (drug effects)
• Kidney (cytology)
• Survival Analysis
• Zebrafish (immunology)
• beta-Glucans (pharmacology)