Abstract |
This study examined the potential of a recombinant marsupial zona pellucida 3 protein as a contraceptive vaccine for the Eastern Grey kangaroo, a marsupial that is locally overabundant in several regions of eastern Australia. First, a pilot study using porcine zona pellucidae (PZP) demonstrated that ZP proteins, primarily the ZP3 component of PZP, are highly immunogenic in the grey kangaroo and produce a long-lasting humoral response to a single immunisation, as found in other marsupials. Immunisation with 300 microg of a non-glycosylated recombinant brushtail possum ZP3 (recBP-ZP3) protein in complete Freund's adjuvant produced a similar, significant and sustained antibody response, and none of the immunised kangaroos (n=7) produced offspring during the following breeding season compared with four out of the six control animals. An epitope analysis of the B-cell response to recBP-ZP3 using a brushtail possum ZP3 identified numerous B-cell epitope regions clustered around the N- and C-terminal regions of the protein. Two regions of interest for further fertility vaccine development based on their immunogenicity and fertility trials and functional studies in other species were found to be immunogenic. These results suggest that immunocontraception based on targeting the ZP3 protein within the zona pellucida may be an effective strategy for fertility reduction in Eastern Grey kangaroos.
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Authors | Anne L Kitchener, Amanda Harman, David J Kay, Carmen A McCartney, Karen E Mate, John C Rodger |
Journal | Journal of reproductive immunology
(J Reprod Immunol)
Vol. 79
Issue 2
Pg. 156-62
(Jan 2009)
ISSN: 1872-7603 [Electronic] Ireland |
PMID | 19215986
(Publication Type: Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Egg Proteins
- Epitopes
- Immune Sera
- Membrane Glycoproteins
- Receptors, Cell Surface
- Recombinant Proteins
- Vaccines, Contraceptive
- Zona Pellucida Glycoproteins
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Topics |
- Animals
- Antibody Formation
(immunology)
- Color
- Contraception, Immunologic
- Egg Proteins
(genetics, immunology, metabolism)
- Epitopes
(immunology)
- Fertilization
(immunology)
- Immune Sera
(immunology)
- Immunization
- Macropodidae
(immunology, physiology)
- Membrane Glycoproteins
(genetics, immunology, metabolism)
- Receptors, Cell Surface
(genetics, immunology, metabolism)
- Recombinant Proteins
(genetics, immunology, metabolism)
- Swine
- Trichosurus
(genetics)
- Vaccines, Contraceptive
(immunology)
- Zona Pellucida
(immunology)
- Zona Pellucida Glycoproteins
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