Protective effect of retinoid against endotoxin-induced mastitis in rats.

Abstract

OBJECTIVE:

A lipopolysaccharide (LPS) induced mastitis model in rats was used to study the protective effect of retinoid.

MATERIALS AND METHODS:

Commencing at gestation day 10, retinoid (dissolved in olive oil) or an equal volume of olive oil was administered to rats daily by gavage until parturition. LPS or pyrogen-free physiological saline was inoculated into the mammary gland 72h after parturition and the rats were euthanized at 12h post-infection.

RESULTS:

Myeloperoxidase (MPO), N-acetyl -beta-D- glucosaminidase (NAGase), tumor necrosis factor-alpha (TNF-alpha), interleukin-8 (IL-8) in mammary tissues and CD4(+)/CD8(+) in peripheral blood were increased and serum MPO and IL-2 in mammary tissues were decreased 12h after LPS infusion. Retinoid decreased MPO, NAGase, and TNF-alpha in mammary tissue and increased IL-2 in serum. Four thousand and 8000 I.U/kg x d of retinoid significantly decreased the infiltration of PMNs in mammary alveoli and ameliorated the imbalance of CD4+/CD8+ in peripheral blood.

CONCLUSION:

These results suggest that retinoid protected against LPS-induced mastitis in a rat model.

• Authors: B-B Gu, J-F Miao, Y-M Zhu, Y-E Deng, S-X Zou
• Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] (Inflamm Res) Vol. 58 Issue 2 Pg. 81-8 (Feb 2009) ISSN: 1420-908X [Electronic] Switzerland
• PMID: 19184358 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Endotoxins
• Interleukin-2
• Interleukin-8
• Lipopolysaccharides
• Retinoids
• Tumor Necrosis Factor-alpha
• Peroxidase
• Acetylglucosaminidase

Topics

• Acetylglucosaminidase (metabolism)
• Animals
• Anti-Inflammatory Agents (metabolism, therapeutic use)
• Endotoxins (adverse effects, immunology)
• Female
• Interleukin-2 (metabolism)
• Interleukin-8 (metabolism)
• Lipopolysaccharides (adverse effects, immunology)
• Male
• Mastitis (chemically induced, drug therapy, pathology, veterinary)
• Peroxidase (metabolism)
• Pregnancy
• Rats
• Rats, Sprague-Dawley
• Retinoids (metabolism, therapeutic use)
• Tumor Necrosis Factor-alpha (metabolism)