[Application of SELDI-TOF-MS in detection of liver metastasis from colorectal cancer].

Abstract

OBJECTIVE:

To establish a serum protein fingerprint model for prediction of liver metastasis from colorectal cancer by SELDI-TOF-MS analysis, and to determine the differentiatial proteins associated with the metastatic liver cancers.

METHODS:

Data were collected from the Department of General Surgery in Zhongshan Hospital. A group of patients with colorectal cancer (CRC) without liver metastasis (n = 36) and another group with liver metastasis (n = 36) were included in this study. Serum samples were collected from peripheral venous blood before operation. Special serum protein or peptide fingerprint was determined by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). The obtained data were analyzed by Biomarker Wizard software to screen the serum protein markers discriminating colorectal cancer patients with and without liver metastasis. A serum protein fingerprint model was established. This model was blindly verified in of CRC patients with and 44 cases without liver metastasis.

RESULTS:

Comparing the characteristic proteins in those two groups of patients, 10 specific protein peaks were identified with statistical significance (P < 0.05). According to m/z growing from small to large, they were: 2398, 2814, 4084, 4289, 4465, 6422, 6619, 11 482, 11 649 and 13 714. The predictive model had a sensitivity of 91.7% and a specificity of 97.2%. The validation showed a sensitivity of 75.0% and a specificity of 81.8%.

CONCLUSION:

A predictive model based on differentiatial serum protein fingerprint with high sensitivity and specificity has been successfully established. It should be a very useful tool in detection and diagnosis of liver metastasis in colorectal cancer patients.

• Authors: Yi-Jiu Shi, Yun Zhao, Jian-Min Xu, Yan-Han Lai, Xin-Zhe Yu, Yun-Shi Zhong, Ye Wei, Li Ren, De-Xiang Zhu, Yin-Kun Liu, Wei-Xin Niu, Xin-Yu Qin
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 30 Issue 12 Pg. 910-3 (Dec 2008) ISSN: 0253-3766 [Print] China
• PMID: 19173991 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Blood Proteins
• Neoplasm Proteins

Topics

• Aged
• Biomarkers, Tumor (blood)
• Blood Proteins (analysis)
• Colorectal Neoplasms (blood, pathology)
• Female
• Humans
• Liver Neoplasms (blood, diagnosis, secondary)
• Male
• Middle Aged
• Neoplasm Proteins (blood)
• Peptide Mapping
• Sensitivity and Specificity
• Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (methods)