MicroRNA-15b regulates cell cycle progression by targeting cyclins in glioma cells.

Abstract

MicroRNAs (miRNAs) are non-protein-coding RNAs that function as post-transcriptional gene regulators. Recent evidence has shown that miRNA plays a pivotal role in the development of many cancers including glioma, a lethal brain cancer. We have recently compared the miRNA expression profiles between normal brain and glioma tissues from Chinese patients by miRNA microarray and identified a panel of differentially expressed miRNAs. Here, we studied the function of one miRNA, miR-15b, in glioma carcinogenesis and elucidated its downstream targets. Over-expression of miR-15b resulted in cell cycle arrest at G0/G1 phase while suppression of miR-15b expression resulted in a decrease of cell populations in G0/G1 and a corresponding increase of cell populations in S phase. We further showed that CCNE1 (encoding cyclin E1) is one of the downstream targets of miR-15b. Taken together, our findings indicate that miR-15b regulates cell cycle progression in glioma cells by targeting cell cycle-related molecules.

• Authors: Hongping Xia, Yanting Qi, Samuel S Ng, Xiaona Chen, Shen Chen, Marong Fang, Dan Li, Yu Zhao, Ruiguang Ge, Guo Li, Yangchao Chen, Ming-Liang He, Hsiang-fu Kung, Lihui Lai, Marie C Lin
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 380 Issue 2 Pg. 205-10 (Mar 06 2009) ISSN: 1090-2104 [Electronic] United States
• PMID: 19135980 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Cyclins
• MIRN15 microRNA, human
• MicroRNAs

Topics

• Brain (metabolism, pathology)
• Brain Neoplasms (genetics, pathology)
• Cell Cycle (genetics)
• Cell Line, Tumor
• Cell Transformation, Neoplastic (genetics, pathology)
• Cyclins (genetics)
• Gene Expression Regulation, Neoplastic
• Glioma (genetics, pathology)
• Humans
• MicroRNAs (genetics, metabolism)