Abstract | BACKGROUND: METHODS: A cell line with stable maspin expression was established. An epithelial growth factor ( EGF)-induced epithelial-mesenchymal transition (EMT) model was used to mimic some aspects of the metastatic process in vitro. The effects of maspin reintroduction on EGF-induced EMT and cell growth characteristics were evaluated. Comparative proteomic analysis of transfected cells versus parental cells was then performed to explore the potential mechanism. RESULTS: The introduction of maspin into EC109 cells was able to inhibit EGF-induced EMT and altered cell growth characteristics, including the serum dependence, proliferative response to EGF stimulation, and colony formation ability in soft agar, indicating a conversion from a malignant phenotype to a benign phenotype. Proteomic analysis revealed a significant down-regulation of a group of glycolytic enzymes in maspin-transfected cells. In addition, maspin-transfected cells expressed much lower levels of hypoxia-inducible factor 1alpha than parental cells or empty vector transfected cells. CONCLUSIONS:
Maspin exhibited a metastasis-suppressive effect, which may be a consequence of the reversal of the malignant phenotype of EC109 cells. The switch of cellular metabolic phenotype to low glycolysis by the gain of maspin function may play a key role in the process. This finding provides additional evidence of the tumor metastasis-suppressive activity of maspin and may indicate a new direction for future studies of the mechanism of maspin.
|
Authors | Zhen Cai, Yuan Zhou, Ting Lei, Jen-Fu Chiu, Qing-Yu He |
Journal | Cancer
(Cancer)
Vol. 115
Issue 1
Pg. 36-48
(Jan 01 2009)
ISSN: 0008-543X [Print] United States |
PMID | 19090015
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright (c) 2008 American Cancer Society. |
Chemical References |
- SERPIN-B5
- Serine Proteinase Inhibitors
- Serpins
- Epidermal Growth Factor
|
Topics |
- Cell Differentiation
- Cell Line, Tumor
- Epidermal Growth Factor
(pharmacology)
- Epithelial Cells
- Esophageal Neoplasms
- Humans
- Neoplasm Metastasis
(prevention & control)
- Serine Proteinase Inhibitors
(metabolism, physiology)
- Serpins
(metabolism, physiology)
- Transfection
|