Characterization of neutralizing epitopes of varicella-zoster virus glycoprotein H.

Abstract

Varicella-zoster virus (VZV) glycoprotein H (gH) is the major neutralization target of VZV, and its neutralizing epitope is conformational. Ten neutralizing human monoclonal antibodies to gH were used to map the epitopes by immunohistochemical analysis and were categorized into seven epitope groups. The combinational neutralization efficacy of two epitope groups was not synergistic. Each epitope was partially or completely resistant to concanavalin A blocking of the glycomoiety of gH, and their antibodies inhibited the cell-to-cell spread of infection. The neutralization epitope comprised at least seven independent protein portions of gH that served as the target to inhibit cell-to-cell spread.

• Authors: Yasushi Akahori, Kazuhiro Suzuki, Tohru Daikoku, Masae Iwai, Yoshihiro Yoshida, Yoshizo Asano, Yoshikazu Kurosawa, Kimiyasu Shiraki
• Journal: Journal of virology (J Virol) Vol. 83 Issue 4 Pg. 2020-4 (Feb 2009) ISSN: 1098-5514 [Electronic] United States
• PMID: 19073736 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Epitopes
• Membrane Glycoproteins
• Viral Proteins
• gp 118 protein, Herpesvirus 3, Human

Topics

• Antibodies, Monoclonal (immunology)
• Antibodies, Viral (immunology)
• Epitope Mapping
• Epitopes (immunology)
• Herpesvirus 3, Human (immunology)
• Humans
• Membrane Glycoproteins (immunology)
• Neutralization Tests
• Viral Proteins (immunology)