New trends in the use of Al(OH)3-conjugated endotoxins and their subunits from the S- and R-forms of Shigella dysenteriae serovar 1 for model vaccination purposes.

Abstract

The lipopolysaccharides, lipopolysaccharide-protein complexes and their lipids A, isolated from Shigella dysenteriae 1, exhibited lethal toxicity (LD50 300-400 micrograms per mouse), pyrogenicity (0.01-1.0 microgram), activity in the Limulus test (10(-3)-10(-12) mg ml-1) and produced a positive local Shwartzman reaction. It was found that these substances bound to Al(OH)3 were capable of successfully protecting mice from challenge with both homologous and heterologous infections (up to 90% of mice survived a challenge with LD100), whereas immunization with substances with no biological carrier exhibited very low or zero protective effects. Furthermore, the positive cross-reactivity of lipids A in the agar immunodiffusion test confirms the wide applicability for vaccination purposes.

• Authors: J Sourek, J Levin, T Trnka, L Zelenková
• Journal: Vaccine (Vaccine) Vol. 9 Issue 2 Pg. 106-10 (Feb 1991) ISSN: 0264-410X [Print] Netherlands
• PMID: 1905447 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Bacterial Vaccines
• Endotoxins
• Pyrogens
• Aluminum Hydroxide

Topics

• Aluminum Hydroxide
• Animals
• Bacterial Vaccines (isolation & purification, toxicity)
• Dysentery, Bacillary (prevention & control)
• Endotoxins (isolation & purification, toxicity)
• Immunodiffusion
• Lethal Dose 50
• Limulus Test
• Mice
• Mice, Inbred ICR
• Pyrogens
• Rabbits
• Shigella dysenteriae (classification, immunology)
• Shwartzman Phenomenon