Abstract |
We have developed an efficient versatile in vivo dendritic cell (DC) targeting vector for delivering different classes of antigens such as proteins, peptide, glycolipids and naked DNA for vaccine applications. A single chain antibody (scFv) that recognizes DEC-205 receptor of DC was fused with a core- streptavidin domain and expressed in Escherichia coli using the T7 expression system. The bifunctional fusion protein (bfFp) was expressed as a periplasmic soluble protein and affinity-purified in its monomeric form. The bifunctional activity against DEC-205 and biotin was characterized by ELISA and Western blot. In vivo DC targeting of a diverse group of biotinylated antigens such as viral and bacterial proteins, a cancer peptide, gangliosides and DNA of certain infectious diseases was conducted in mice. Results show that in the presence of bfFp and costimulatory anti-CD40 mAb, both humoral and cell-mediated responses were augmented in either the single antigen or multiple antigen targeting strategy. Lastly, bfFp based DC targeting of antigens in low doses may be a useful strategy for the design of monovalent or polyvalent vaccines for the masses.
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Authors | Welson W Wang, Dipankar Das, Mavanur R Suresh |
Journal | Molecular pharmaceutics
(Mol Pharm)
2009 Jan-Feb
Vol. 6
Issue 1
Pg. 158-72
ISSN: 1543-8384 [Print] United States |
PMID | 19053535
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Bacterial Vaccines
- Haptens
- Recombinant Proteins
- Viral Vaccines
- Biotin
- Interferon-gamma
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Topics |
- Animals
- Antibodies
(genetics, immunology, isolation & purification, metabolism)
- Bacterial Vaccines
(genetics, metabolism)
- Biotin
- Cell Line
- Dendritic Cells
(immunology, metabolism)
- Female
- Gene Expression
- Genetic Vectors
(genetics)
- Haptens
(immunology)
- Interferon-gamma
(metabolism)
- Mice
- Mice, Inbred BALB C
- Rats
- Recombinant Proteins
(genetics, isolation & purification, metabolism)
- Viral Vaccines
(genetics, metabolism)
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