Identification of different
protein functions facilitates a mechanistic understanding of Japanese encephalitis virus (JEV)
infection and opens novel means for drug development. Support vector machines (SVM), useful for predicting the functional class of distantly related
proteins, is employed to ascribe a possible functional class to Japanese encephalitis virus
protein. Our study from SVMProt and available JE virus sequences suggests that structural and nonstructural
proteins of JEV genome possibly belong to diverse
protein functions, are expected to occur in the life cycle of JE virus.
Protein functions common to both structural and non-structural
proteins are
iron-binding,
metal-binding,
lipid-binding,
copper-binding, transmembrane, outer
membrane, channels/Pores - Pore-forming toxins (
proteins and
peptides) group of
proteins. Non-structural
proteins perform functions like actin binding,
zinc-binding,
calcium-binding,
hydrolases,
Carbon-Oxygen Lyases,
P-type ATPase,
proteins belonging to major facilitator family (MFS), secreting main terminal branch (MTB) family, phosphotransfer-driven group translocators and
ATP-binding cassette (ABC) family group of
proteins. Whereas structural
proteins besides belonging to same structural group of
proteins (capsid, structural, envelope), they also perform functions like
nuclear receptor, antibiotic resistance,
RNA-binding,
DNA-binding,
magnesium-binding,
isomerase (intra-molecular),
oxidoreductase and participate in type II (general) secretory pathway (IISP).