Imatinib inhibits in vitro proliferation of cells derived from a pleural solitary fibrous tumor expressing platelet-derived growth factor receptor-beta.

Abstract

We examined the in vitro effects of imatinib (Novartis Pharma AG, Basel, Switzerland) as a possible inhibitor of PDGFR pathway on cells derived from a recurrence of a pleural malignant solitary fibrous tumor (SFT). Primary cell culture was characterised by immunofluorescence. SFT-derived cells were treated with imatinib at different time points. Western blotting for PDGFR-beta, phospho-PDGFR-beta or smooth muscle actin (SMA) was performed before and after 96 h of treatment with imatinib. SFT-derived cells treated with imatinib for 96 h showed a dose dependent decrease of Ki67 expression. Results were confirmed by growth curve. Western blotting showed that PDGFR-beta was highly expressed and phosphorylated in SFT-derived cells and imatinib treatment reduced PDGFR-beta phosphorylation and SMA expression. With the limit of experimental findings, our results support a possible future application of imatinib as a candidate molecule in the target therapy of malignant SFTs over-expressing wild-type PDGFR.

• Authors: Marco Prunotto, Martino Bosco, Lorenzo Daniele, Luigia Macri', Lisa Bonello, Laura Schirosi, Giulio Rossi, Pierluigi Filosso, Baudolino Mussa, Anna Sapino
• Journal: Lung cancer (Amsterdam, Netherlands) (Lung Cancer) Vol. 64 Issue 2 Pg. 244-6 (May 2009) ISSN: 1872-8332 [Electronic] Ireland
• PMID: 19041155 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Benzamides
• Piperazines
• Pyrimidines
• Imatinib Mesylate
• Receptor, Platelet-Derived Growth Factor alpha
• Receptor, Platelet-Derived Growth Factor beta
• Cisplatin
• Fluorouracil

Topics

• Aged
• Antineoplastic Agents (pharmacology)
• Antineoplastic Combined Chemotherapy Protocols (therapeutic use)
• Benzamides
• Blotting, Western
• Cell Proliferation (drug effects)
• Cells, Cultured
• Cisplatin (administration & dosage)
• Female
• Fluorescent Antibody Technique
• Fluorouracil (administration & dosage)
• Humans
• Imatinib Mesylate
• In Vitro Techniques
• Neoplasm Recurrence, Local (metabolism, pathology)
• Piperazines (pharmacology)
• Pneumonectomy
• Pyrimidines (pharmacology)
• Radiotherapy
• Receptor, Platelet-Derived Growth Factor alpha (biosynthesis)
• Receptor, Platelet-Derived Growth Factor beta (biosynthesis, drug effects)
• Solitary Fibrous Tumor, Pleural (metabolism, pathology, therapy)