Promotion of the self-renewal capacity of human acute leukemia cells by Wnt3A.

Abstract

BACKGROUND:

Wnt/beta-catenin signaling is involved in the growth of various types of cancer cells. Wnt3A has been reported to promote the self-renewal of hematopoietic stem cells.

MATERIALS AND METHODS:

The effects of recombinant Wnt3A protein on the in vitro growth of four acute myeloid leukemia (AML) and four acute T-lymphoblastic leukemia (T-ALL) cell lines was examined.

RESULTS:

Wnt3A stimulation either had no effect on, or slightly suppressed, the short-term growth of these cell lines. In three cell lines, Wnt3A promoted clonogenic cell recovery after suspension culture, suggesting the promotion of the self-renewal capacity of leukemic stem or progenitor cells. Immunoblot analysis showed that Wnt3A stimulation reduced phosphorylated beta-catenin and increased beta-catenin in these cells, indicating that Wnt3A stimulation activated Wnt/beta-catenin signaling.

CONCLUSION:

Wnt3A stimulation did not promote the growth of whole cell populations, but did promote the self-renewal of leukemic stem/progenitor cells in some AML and T-ALL cell lines.

• Authors: Noriko Kawaguchi-Ihara, Ikuo Murohashi, Nobuo Nara, Shuji Tohda
• Journal: Anticancer research (Anticancer Res) 2008 Sep-Oct Vol. 28 Issue 5A Pg. 2701-4 ISSN: 0250-7005 [Print] Greece
• PMID: 19035298 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD34
• Calcium-Binding Proteins
• Intercellular Signaling Peptides and Proteins
• Membrane Proteins
• NOTCH1 protein, human
• RNA, Messenger
• Receptor, Notch1
• Recombinant Proteins
• Serrate-Jagged Proteins
• WNT3A protein, human
• Wnt Proteins
• Wnt3 Protein
• Wnt3A Protein
• ADP-ribosyl Cyclase 1

Topics

• ADP-ribosyl Cyclase 1 (biosynthesis)
• Acute Disease
• Antigens, CD34 (biosynthesis)
• Calcium-Binding Proteins (biosynthesis, genetics)
• Cell Growth Processes (drug effects)
• Cell Line, Tumor
• Gene Expression (drug effects)
• Humans
• Intercellular Signaling Peptides and Proteins (biosynthesis, genetics)
• Leukemia, Myeloid (drug therapy, genetics, metabolism, pathology)
• Membrane Proteins (biosynthesis, genetics)
• Neoplastic Stem Cells (drug effects)
• Precursor T-Cell Lymphoblastic Leukemia-Lymphoma (drug therapy, genetics, metabolism, pathology)
• RNA, Messenger (biosynthesis, genetics)
• Receptor, Notch1 (biosynthesis, genetics)
• Recombinant Proteins (pharmacology)
• Serrate-Jagged Proteins
• Wnt Proteins (pharmacology)
• Wnt3 Protein
• Wnt3A Protein