Abstract | BACKGROUND: MATERIAL AND METHODS: The human breast cancer cell line MDA-MB-231 and MDA-MB-231 transfectedp27Kip1 MDA-MB-p27 were used for the invasion assay, Western blotting and real-time quantitative RT-PCR. RESULTS: In the invasion assay, the invasion of MDA-MB-p27 was significantly less than that of the parent cell line. In Western blotting analyses, the protein level of MMP-9 was also reduced in MDA-MB-p27. Furthermore, the activity of MMP-9 in cell culture supernatants was lower in MDA-MB-p27 as compared with enzyme-linked immunosorbent assays. In real-time quantitative RT-PCR, the mRNA level of MMP-9 was lower in MDA-MB-p27 cells. CONCLUSION: Up-regulation of p27Kip1 remarkably inhibited the invasion of the breast cancer cells, in part due to the reduced expression of MMP-9. This is the first report of p27Kip1 modulating MMP-9 and indicating that p27Kip1 might play a key role in tumor cell invasion.
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Authors | Masamichi Mizuma, Yu Katayose, Kuniharu Yamamoto, Satoru Shiraso, Tsuyoshi Sasaki, Shinichi Yabuuchi, Akira Oda, Kunihiro Masuda, Toshiki Rikiyama, Tohru Onogawa, Hideo Ohtsuka, Fuyuhiko Motoi, Shinichi Egawa, Michiaki Unno |
Journal | Anticancer research
(Anticancer Res)
2008 Sep-Oct
Vol. 28
Issue 5A
Pg. 2669-77
ISSN: 0250-7005 [Print] Greece |
PMID | 19035293
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CDKN1B protein, human
- Intracellular Signaling Peptides and Proteins
- RNA, Messenger
- Retinoblastoma Protein
- Cyclin-Dependent Kinase Inhibitor p27
- Matrix Metalloproteinase 9
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Topics |
- Breast Neoplasms
(genetics, metabolism, pathology)
- Cell Cycle
(physiology)
- Cell Growth Processes
(physiology)
- Cell Line, Tumor
- Cyclin-Dependent Kinase Inhibitor p27
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics, metabolism)
- Matrix Metalloproteinase 9
(biosynthesis, metabolism)
- Neoplasm Invasiveness
- RNA, Messenger
(biosynthesis, genetics)
- Retinoblastoma Protein
(biosynthesis, genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Up-Regulation
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