Abstract |
A 49-year-old man consumed two glasses (approximately 2 x 20 mL) of a beverage containing yellow gentian (Gentiana lutea). Shortly after ingestion, he developed nausea, vomiting, and oral paraesthesia. On admission to the hospital he suffered from severe bradycardia (35 beats/min) and hypotension (50/30 mm Hg), and he was treated with activated charcoal, antiemetics ( metoclopramide, ondansetron), atropine, and intravenous electrolytic solution. The initial suspicion of Veratrum poisoning could be confirmed by identifying protoveratrines A (ProA) and protoveratrine B (ProB) in a sample from the beverage as well as in the patients serum by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS-MS). The yellow-colored beverage contained 25% ethanol (by headspace gas chromatography), 20.4 mg/L ProA, and 13.7 mg/L ProB. The serum concentration of ProA was 1162 ng/L and ProB was 402 ng/L. Veratridine, cevadine, and jervine were not detected, neither in the beverage nor in the serum sample. The lower limits of quantitation for all compounds is 10 microg/L (S/N > 10, beverage) and 100 ng/L (S/N > 10, serum). After treatment, the patient completely recovered from the symptoms within 24 h and was discharged from the hospital. The analytical method described was developed for the simultaneous identification and quantitation of five Veratrum alkaloids. The method is based on a liquid-liquid extraction followed by LC-MS-MS analysis. The time needed for analysis was 6 min.
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Authors | T Grobosch, T Binscheck, F Martens, D Lampe |
Journal | Journal of analytical toxicology
(J Anal Toxicol)
2008 Nov-Dec
Vol. 32
Issue 9
Pg. 768-73
ISSN: 0146-4760 [Print] England |
PMID | 19021933
(Publication Type: Case Reports, Journal Article)
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Chemical References |
- Antidotes
- Antiemetics
- Indicators and Reagents
- Veratrum Alkaloids
- Charcoal
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Topics |
- Accidents
- Alcoholic Beverages
(analysis, poisoning)
- Antidotes
(therapeutic use)
- Antiemetics
(therapeutic use)
- Charcoal
(therapeutic use)
- Chromatography, High Pressure Liquid
- Chromatography, Thin Layer
- Gentiana
- Hemodynamics
(drug effects)
- Humans
- Indicators and Reagents
- Male
- Mass Spectrometry
- Middle Aged
- Spectrometry, Mass, Electrospray Ionization
- Spectrophotometry, Ultraviolet
- Veratrum
(chemistry, poisoning)
- Veratrum Alkaloids
(analysis, poisoning)
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