Analysis of SOX9 expression in colorectal cancer.

Abstract

Our purpose was to investigate the role of SOX9, a novel downstream molecule of beta-catenin, in colorectal cancer. Expression of SOX9 and beta-catenin was detected by immunostaining, quantitative real-time reverse transcription-polymerase chain reaction (Q-PCR), and Western blot in colorectal cancer. The correlation between SOX9 or beta-catenin expression and clinicopathologic parameters was also analyzed. Immunostaining, Q-PCR, and Western blot consistently confirmed SOX9 up-regulation in colorectal cancer compared with normal mucosa (P < .05). Immunostaining showed more SOX9+ cells in the lower zone of colonic crypts than in the upper zone (P < .05). Cancers with strong SOX9 immunostaining were significantly associated with a lower 5-year overall survival (40% [17/43] vs low expression, 69% [66/95]; P < .01). The Cox proportional hazards model showed that strong SOX9 expression was an independent adverse prognosticator in colorectal cancer (P < .05). The detection of SOX9 expression might contribute to predicting clinical outcomes for patients with colorectal cancer.

• Authors: Bingjian Lü, Yihu Fang, Jing Xu, Lipei Wang, Fangying Xu, Enping Xu, Qiong Huang, Maode Lai
• Journal: American journal of clinical pathology (Am J Clin Pathol) Vol. 130 Issue 6 Pg. 897-904 (Dec 2008) ISSN: 1943-7722 [Electronic] England
• PMID: 19019766 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• SOX9 Transcription Factor
• SOX9 protein, human
• beta Catenin

Topics

• Adenoma (genetics, metabolism, mortality, pathology)
• Aged
• Blotting, Western
• China (epidemiology)
• Colorectal Neoplasms (genetics, metabolism, mortality, pathology)
• Female
• Gene Expression
• Humans
• Kaplan-Meier Estimate
• Male
• Middle Aged
• Prognosis
• Proportional Hazards Models
• Reverse Transcriptase Polymerase Chain Reaction
• SOX9 Transcription Factor (biosynthesis, genetics, physiology)
• Up-Regulation
• beta Catenin (biosynthesis)