Abstract | OBJECTIVES: METHODS: RESULTS:
Hematuria and urinary bladder edema was observed in the CP-treated rats and histologically, moderate to severe damage to the urinary bladder was observed. The bladders of CP-treated rats stained strongly for nitrotyrosine as well as for PARP. Significant decrease in oxidized NAD levels was observed in the bladders of CP-treated rats 16 h following treatment with CP. Protein thiol was depleted and the activity of the peroxynitrite sensitive enzyme SOD was significantly reduced in the bladders of CP-treated rats. CONCLUSION: The results of the present study reveal that protein nitration, PARP activation and NAD+ depletion may play a critical role in the pathogenesis of CP-induced hemorrhagic cystitis. Based on the results we propose a mechanism for CP-induced cystitis.
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Authors | Premila Abraham, Suganthy Rabi |
Journal | Cancer chemotherapy and pharmacology
(Cancer Chemother Pharmacol)
Vol. 64
Issue 2
Pg. 279-85
(Jul 2009)
ISSN: 1432-0843 [Electronic] Germany |
PMID | 19015854
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Alkylating
- Antioxidants
- NAD
- Peroxynitrous Acid
- Nitric Oxide
- 3-nitrotyrosine
- Tyrosine
- Malondialdehyde
- Cyclophosphamide
- Superoxide Dismutase
- Poly(ADP-ribose) Polymerases
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Topics |
- Animals
- Antineoplastic Agents, Alkylating
(adverse effects)
- Antioxidants
(pharmacology)
- Cyclophosphamide
(adverse effects)
- Cystitis
(chemically induced, metabolism, pathology)
- Hemorrhage
(chemically induced, metabolism, pathology)
- Immunoenzyme Techniques
- Male
- Malondialdehyde
(metabolism)
- NAD
(metabolism)
- Nitric Oxide
(metabolism)
- Oxidative Stress
- Peroxynitrous Acid
(metabolism)
- Poly(ADP-ribose) Polymerases
(metabolism)
- Rats
- Rats, Wistar
- Superoxide Dismutase
(metabolism)
- Tyrosine
(analogs & derivatives, metabolism)
- Urinary Bladder
(drug effects, metabolism, pathology)
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