Abstract | OBJECTIVES: METHODS: Vascular smooth muscle cells from thoracic aorta of adult Wistar rats were cultured and subjected to hypoxia at 2.5% oxygen in a hypoxic chamber. Western blot, real-time PCR, reactive oxygen species assay, and promoter activity were measured. RESULTS:
Hypoxia significantly increased the resistin protein (3.5-fold, P < 0.001) and mRNA (4.8-fold, P < 0.001) expression as compared with the control cells. The specific extracellular signal-regulated kinase (ERK) inhibitor PD98059, antioxidant N-acetylcysteine, and ERK siRNA attenuated the induction of resistin protein by hypoxia. It increased the phosphorylated ERK protein expression (3.2-fold, P < 0.001), whereas pretreatment with PD98059 and N-acetylcysteine significantly blocked the increase of phosphorylated ERK by hypoxia. It also increased the reactive oxygen species production (9.3-fold, P < 0.001), and pretreatment with N-acetylcysteine significantly blocked the induction of reactive oxygen species by hypoxia. Hypoxia increased resistin promoter activity (5.1-fold, P < 0.001), and the activity was abolished when nuclear factor of activating T cells in the promoter area was mutated. Pretreatment with PD98059 and N-acetylcysteine significantly attenuated the resistin promoter activity induced by hypoxia. CONCLUSION:
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Authors | Huei-Fong Hung, Bao-Wei Wang, Hang Chang, Kou-Gi Shyu |
Journal | Journal of hypertension
(J Hypertens)
Vol. 26
Issue 12
Pg. 2349-60
(Dec 2008)
ISSN: 0263-6352 [Print] Netherlands |
PMID | 19008713
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- NFATC Transcription Factors
- RNA, Messenger
- Reactive Oxygen Species
- Resistin
- Extracellular Signal-Regulated MAP Kinases
- Glucose
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Topics |
- Animals
- Aorta, Thoracic
(cytology, metabolism)
- Cell Hypoxia
(physiology)
- Cells, Cultured
- Extracellular Signal-Regulated MAP Kinases
(genetics, metabolism)
- Glucose
(metabolism)
- Male
- Models, Animal
- Muscle, Smooth, Vascular
(cytology, metabolism)
- NFATC Transcription Factors
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Resistin
(genetics, metabolism)
- Signal Transduction
(physiology)
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