Abstract | BACKGROUND: OBJECTIVE: We sought to gain further understanding of the genetic determinants of plasma folate and homocysteine concentrations. Because folate is required for the synthesis of thymidine from uracil, the latter accumulating and being misincorporated into DNA during folate depletion, the DNA uracil content was also measured. DESIGN: RESULTS: The MTHFR 677TT genotype was associated with increased plasma homocysteine and decreased plasma folate. MTHFR 1298A>C and RFC1 intron 5A>G polymorphisms were associated with significantly altered plasma homocysteine concentrations. The FOLH1 1561C>T SNP was associated with altered plasma folate concentrations. The MTHFR 677TT genotype was associated with a approximately 34% lower DNA uracil content (P = 0.045), whereas the G allele of the GGH -124T>G SNP was associated with a stepwise increase in DNA uracil content (P = 0.022). CONCLUSION: Because the accumulation of uracil in DNA induces chromosome breaks, mutagenic lesions, we suggest that, as for MTHFR C677T, the GGH -124 T>G SNP may modulate the risk of carcinogenesis and therefore warrants further attention.
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Authors | Lauren DeVos, Aurelie Chanson, Zhenhua Liu, Eric D Ciappio, Laurence D Parnell, Joel B Mason, Katherine L Tucker, Jimmy W Crott |
Journal | The American journal of clinical nutrition
(Am J Clin Nutr)
Vol. 88
Issue 4
Pg. 1149-58
(Oct 2008)
ISSN: 1938-3207 [Electronic] United States |
PMID | 18842806
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Membrane Transport Proteins
- Homocysteine
- Uracil
- Folic Acid
- Methylenetetrahydrofolate Reductase (NADPH2)
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Topics |
- Aged
- Chromosome Aberrations
- Cohort Studies
- DNA Damage
(genetics)
- DNA Methylation
- Female
- Folic Acid
(blood, metabolism)
- Gene Expression Regulation, Enzymologic
- Homocysteine
(blood)
- Humans
- Male
- Membrane Transport Proteins
(genetics)
- Methylenetetrahydrofolate Reductase (NADPH2)
(genetics)
- Middle Aged
- Polymorphism, Single Nucleotide
- Risk Factors
- Uracil
(metabolism)
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