Cyanidin 3-O-glucopyranoside protects and rescues SH-SY5Y cells against amyloid-beta peptide-induced toxicity.

Abstract

The amyloid-beta (A beta) peptide (1-42) aggregation into oligomeric and fibrillar species affects neuronal viability, having a causal role in the development of Alzheimer's disease. Among dietary anthocyanins, cyanidin 3-O-glucoside (Cy-3G) and its metabolites, such as protocatechuic acid (PA), have gained attention as potential neuroprotective agents. In this in-vitro study, we demonstrated that Cy-3G, but not PA, can inhibit A beta1-42 spontaneous aggregation using thioflavin T fluorescence assay and transmission electron microscopy. Furthermore, treatment of human neuronal SH-SY5Y cells with Cy-3G during oligomeric and fibrillar A beta1-42 treatment prevents neuronal viability loss. These protective effects were still evident when Cy-3G treatment was initiated after the appearance of oligomeric A beta1-42 neurotoxicity. Taken together, these results suggest that Cy-3G may protect and rescue the neuronal cells from toxicity induced by A beta1-42.

• Authors: Andrea Tarozzi, Adriana Merlicco, Fabiana Morroni, Francesca Franco, Giorgio Cantelli-Forti, Gabriella Teti, Mirella Falconi, Patrizia Hrelia
• Journal: Neuroreport (Neuroreport) Vol. 19 Issue 15 Pg. 1483-6 (Oct 08 2008) ISSN: 1473-558X [Electronic] England
• PMID: 18797302 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid beta-Peptides
• Anthocyanins
• Benzothiazoles
• Glucosides
• Neuroprotective Agents
• Peptide Fragments
• Thiazoles
• cyanidin-3-O-beta-glucopyranoside
• thioflavin T

Topics

• Amyloid beta-Peptides (chemistry, toxicity)
• Anthocyanins (pharmacology)
• Apoptosis (drug effects)
• Benzothiazoles
• Cell Death (drug effects)
• Cell Line, Tumor
• Drug Evaluation, Preclinical (methods)
• Glucosides (pharmacology)
• Humans
• Microscopy, Electron, Transmission
• Microscopy, Fluorescence (methods)
• Neurons (drug effects, pathology, ultrastructure)
• Neuroprotective Agents (pharmacology)
• Peptide Fragments (toxicity)
• Thiazoles (chemistry)