Cellular protection of morin against the oxidative stress induced by hydrogen peroxide.

Abstract

Flavonoids are a class of secondary metabolites abundantly found in fruits and vegetables. In addition, flavonoids have been reported as potent antioxidants with beneficial effects against oxidative stress-related diseases such as cancer, aging, and diabetes. The present study was carried out to investigate the cytoprotective effects of morin (2',3,4',5,7-pentahydroxyflavone), a member of the flavonoid group, against hydrogen peroxide (H(2)O(2))-induced DNA and lipid damage. Morin was found to prevent the cellular DNA damage induced by H(2)O(2) treatment, which is shown by the inhibition of 8-hydroxy-2'-deoxyguanosine (8-OHdG) formation (a modified form of DNA base), inhibition of comet tail (a form of DNA strand breakage), and decrease of nuclear phospho histone H2A.X expression (a marker for DNA strand breakage). In addition, morin inhibited membrane lipid peroxidation, which is detected by inhibition of thiobarbituric acid reactive substance (TBARS) formation. Morin was found to scavenge the intracellular reactive oxygen species (ROS) generated by H(2)O(2) treatment in cells, which is detected by a spectrofluorometer, flow cytometry, and confocal microscopy after staining of 2',7'-dichlorodihydrofluorescein diacetate (DCF-DA). Morin also induces an increase in the activity of catalase and protein expression. The results of this study suggest that morin protects cells from H(2)O(2)-induced damage by inhibiting ROS generation and by inducing catalase activation.

• Authors: Rui Zhang, Kyoung Ah Kang, Mei Jing Piao, Young Hee Maeng, Keun Hwa Lee, Weon Young Chang, Ho Jin You, Ju Sun Kim, Sam Sik Kang, Jin Won Hyun
• Journal: Chemico-biological interactions (Chem Biol Interact) Vol. 177 Issue 1 Pg. 21-7 (Jan 15 2009) ISSN: 1872-7786 [Electronic] Ireland
• PMID: 18793623 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Flavonoids
• Reactive Oxygen Species
• Thiobarbituric Acid Reactive Substances
• morin
• Hydrogen Peroxide
• Catalase

Topics

• Animals
• Catalase (metabolism)
• Cell Line
• Cricetinae
• Cricetulus
• Cytoprotection (drug effects)
• DNA Damage
• Flavonoids (chemistry, pharmacology)
• Hydrogen Peroxide (pharmacology)
• Lipid Peroxidation (drug effects)
• Oxidative Stress (drug effects)
• Reactive Oxygen Species (metabolism)
• Thiobarbituric Acid Reactive Substances (metabolism)