Lack of toxicity of a STAT3 decoy oligonucleotide.

Abstract

BACKGROUND:

STAT3 overexpression has been detected in several cancers including head and neck squamous cell carcinoma (HNSCC). Previous studies using intratumoral administration of a STAT3 decoy oligonucleotide that abrogates STAT3-mediated gene transcription in preclinical cancer models have demonstrated antitumor efficacy. This study was conducted to observe the toxicity and biologic effects of the STAT3 decoy in a non-human primate model, in anticipation of initiating a clinical trial in HNSCC patients.

METHODS:

Three study groups (two monkeys/sex/group) were administered a single intramuscular injection of low dose of STAT3 decoy (0.8 mg total dose/monkey), high dose of STAT3 decoy (3.2 mg total dose/monkey) or vehicle control (PBS alone) on day 1 and necropsies were performed on days 2 and 15 (one monkey/sex/group/day). Low and high doses of the decoy were administered in the muscle in a volume of 0.9 ml. Tissue and blood were harvested for toxicology and biologic analyses.

RESULTS:

Upon observation, the STAT3 decoy-treated animals exhibited behavior that was similar to the vehicle control group. Individual animal body weights remained within 1% of pretreatment weights throughout the study. Hematological parameters were not significantly different between the control and the treatment groups. Clinical chemistry fluctuations were considered within normal limits and were not attributed to the STAT3 decoy. Assessment of complement activation breakdown product (Bb) levels demonstrated no activation of the alternative pathway of complement in any animal at any dose level. At necropsy, there were no gross or microscopic findings attributed to STAT3 decoy in any organ examined. STAT3 target gene expression at the injection site revealed decreased Bcl-X(L) and cyclin D1 expression levels in the animals treated with high dose of STAT3 decoy compared to the animals injected with low dose of STAT3 decoy or the vehicle as control.

CONCLUSION:

Based on these findings, the no-observable-adverse-effect-level (NOAEL) was greater than 3.2 mg/kg when administered as a single dose to male and female Cynomolgus monkeys. Plans are underway to test the safety and biologic effects of intratumoral administration of the STAT3 decoy in HNSCC patients.

• Authors: Malabika Sen, Patricia J Tosca, Christa Zwayer, Michael J Ryan, Jerry D Johnson, Katherine A B Knostman, Patricia C Giclas, James O Peggins, Joseph E Tomaszewski, Timothy P McMurray, Changyou Li, Michael S Leibowitz, Robert L Ferris, William E Gooding, Sufi M Thomas, Daniel E Johnson, Jennifer R Grandis
• Journal: Cancer chemotherapy and pharmacology (Cancer Chemother Pharmacol) Vol. 63 Issue 6 Pg. 983-95 (May 2009) ISSN: 1432-0843 [Electronic] Germany
• PMID: 18766340 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Oligonucleotides
• STAT3 Transcription Factor
• Tubulin
• bcl-X Protein
• Cyclin D1

Topics

• Animals
• Cyclin D1 (genetics)
• Female
• Gene Expression (drug effects)
• Injections, Intramuscular
• Macaca fascicularis
• Male
• No-Observed-Adverse-Effect Level
• Oligonucleotides (administration & dosage, toxicity)
• STAT3 Transcription Factor (antagonists & inhibitors, genetics, physiology)
• Toxicity Tests
• Tubulin (genetics)
• bcl-X Protein (genetics)