Marmorin, a new ribosome inactivating protein with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the mushroom Hypsizigus marmoreus.

Abstract

Ribosome inactivating proteins (RIPs) are enzymes that inactivate ribosomes by eliminating one or more adenosine residues from rRNA, a 9,567-Da RIP with a novel N-terminal sequence was isolated from fresh fruiting bodies of the mushroom Hypsizigus marmoreus. The protein was unadsorbed on DEAE-cellulose, adsorbed on Affi-gel blue gel, and appeared as a single peak upon gel filtration on Superdex 75. The protein, designated as marmorin, inhibited proliferation of hepatoma Hep G2 cells and breast cancer MCF-7 cells, HIV-1 reverse transcriptase activity, and translation in the rabbit reticulocyte lysate system with an IC50 of 0.15 microM, 5 microM, 30 microM, and 0.7 nM, respectively. Compared to RIPs from hairy gourd, bitter gourd, ridge gourd, garden pea, and the mushroom Flammulina velutipes, marmorin was more potent in its antiproliferative activity toward hepatoma (HepG2) and breast cancer (MCF-7) cells, similar in inhibitory potency toward HIV-1 reverse transcriptase (with the exception that it was more potent than ridge gourd RIP and bitter gourd RIP), and less potent in translation-inhibitory potency. Marmorin was devoid of antifungal, protease, RNase, mitogenic, anti-mitogenic, nitric oxide-inducing, hemagglutinating, and trypsin inhibitory activities.

• Authors: Jack H Wong, H X Wang, T B Ng
• Journal: Applied microbiology and biotechnology (Appl Microbiol Biotechnol) Vol. 81 Issue 4 Pg. 669-74 (Dec 2008) ISSN: 1432-0614 [Electronic] Germany
• PMID: 18751697 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-HIV Agents
• Fungal Proteins
• Growth Inhibitors
• Reverse Transcriptase Inhibitors
• marmorin protein, Hypsizigus marmoreus
• reverse transcriptase, Human immunodeficiency virus 1
• HIV Reverse Transcriptase
• Ribosome Inactivating Proteins

Topics

• Agaricales (chemistry, metabolism)
• Amino Acid Sequence
• Animals
• Anti-HIV Agents (chemistry, isolation & purification, pharmacology)
• Cell Line, Tumor
• Fruiting Bodies, Fungal (chemistry, metabolism)
• Fungal Proteins (chemistry, isolation & purification, pharmacology)
• Growth Inhibitors (chemistry, isolation & purification, pharmacology)
• HIV Reverse Transcriptase (antagonists & inhibitors, metabolism)
• Humans
• Mice
• Molecular Sequence Data
• Molecular Weight
• Momordica charantia (chemistry)
• Reverse Transcriptase Inhibitors (chemistry, isolation & purification, pharmacology)
• Ribosome Inactivating Proteins (chemistry, isolation & purification, pharmacology)
• Sequence Alignment