Application of quantitative gene expression analysis for pertussis vaccine safety control.

Abstract

Although vaccines are routinely used to prevent infectious diseases, little is known about the comprehensive influences caused by vaccines. In this study, we showed, using comprehensive gene expression analysis, that pertussis vaccine affected many genes in multiple organs of vaccine-treated animals. In particular, lung was revealed to be the most suitable target to evaluate pertussis vaccine toxicity. The 13 genes identified from the analysis of vaccine-treated lung at day 1 showed a clear dendrogram corresponding to pertussis vaccine toxicity. Furthermore, quantitative analysis of these genes revealed a positive correlation between their respective expression levels and the degree of toxic effects observed in samples that had been treated with various doses of reference pertussis vaccines. The quantification of this 13 gene-set is an indicator of the vaccine toxicity-related reaction.

• Authors: Isao Hamaguchi, Jun-ichi Imai, Haruka Momose, Mika Kawamura, Takuo Mizukami, Seishiro Naito, Jun-ichi Maeyama, Atsuko Masumi, Madoka Kuramitsu, Kazuya Takizawa, Hiroshi Kato, Tetsuya Mizutani, Yoshinobu Horiuchi, Nobuo Nomura, Shinya Watanabe, Kazunari Yamaguchi
• Journal: Vaccine (Vaccine) Vol. 26 Issue 36 Pg. 4686-96 (Aug 26 2008) ISSN: 0264-410X [Print] Netherlands
• PMID: 18619509 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Pertussis Vaccine

Topics

• Animals
• Body Weight
• Gene Expression Profiling (methods)
• Lung (drug effects)
• Male
• Oligonucleotide Array Sequence Analysis (methods)
• Pertussis Vaccine (adverse effects, toxicity)
• Rats
• Rats, Wistar
• Up-Regulation