Abstract | INTRODUCTION: THE AIM OF THE STUDY: The aim of the study was to examine the effect of MEL on serum and erythrocyte GSH-Px activity after ADR in normal and pinealectomised rats. MATERIAL AND METHODS: Wistar rats were divided into the three groups: control animals (Intact), sham-operated ( Sham-PX) and pinealectomised (Px). Each of the groups was divided into four subgroups, injected with: 1--saline, 2--MEL, 3--ADR and 4--ADR + MEL. ADR was administered 2 months after Px as a single dose (15 mg/kg, i.p.) 1 hour after the fourth melatonin injection. Melatonin (5 mg/kg, i.p.) was administered for 4 days before and 2 days after ADR. After 6 days of treatment, the rats were killed by decapitation. Their blood was collected for measurements. RESULTS: In serum GSH-Px activity decreased in all the groups after ADR. Pinealectomy decreased the activity of the enzyme in all the groups of animals examined. In erythrocytes GSH-Px decreased after ADR in the Px-animals. The effect of pinealectomy on erythrocyte GSH-Px activity was not as strongly expressed as serum GSH-Px activity. MEL did not change GSH-Px activity after ADR. CONCLUSION:
Melatonin, in pharmacological concentrations, did not influence the activity of GSH-Px, either in normal or in pinealectomised rats after ADR. A deficiency of endogenous melatonin production may inhibit GSH-Px activity.
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Authors | Katarzyna Dbrowska, Michał Stuss, Jolanta Gromadzińska, Wojciech Wasowicz, Ewa Sewerynek |
Journal | Endokrynologia Polska
(Endokrynol Pol)
2008 May-Jun
Vol. 59
Issue 3
Pg. 200-6
ISSN: 0423-104X [Print] Poland |
PMID | 18615393
(Publication Type: Journal Article)
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Chemical References |
- Antioxidants
- Doxorubicin
- Glutathione Peroxidase
- Melatonin
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Topics |
- Animals
- Antioxidants
(pharmacology)
- Doxorubicin
(pharmacology)
- Erythrocytes
(metabolism)
- Glutathione Peroxidase
(blood)
- Male
- Melatonin
(pharmacology)
- Pineal Gland
(metabolism, surgery)
- Rats
- Rats, Wistar
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