Abstract |
The present study investigated the chemopreventive effect of dietary fish oil ( Maxepa), rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) on induction of apoptosis in mammary carcinogenesis model. Mammary carcinogenesis was initiated by a single, tail vein injection of 7,12-dimethylbenz(alpha)anthracene (DMBA) (0.5mg/0.2ml corn oil/100g body weight) at 7 weeks of animal age. Ninety female Sprague-Dawley rats were divided into two parts: part one was used for histology and immunohistochemical study and part two for morphological analysis. Each part consists of three experimental groups having 15 animals, i.e., Group A (DMBA control), Group B (DMBA+fish oil) and Group C (DMBA+corn oil). Rats were fed either fish oil or corn oil (0.5ml/day/rat) by oral gavage, 2 weeks prior to DMBA injection. Treatment was continued 25 weeks, studying histopathology, expression of Bax and Bcl-2 proteins by immunohistochemistry and apoptosis by TUNEL assay and morphological study at 36 weeks. Results showed that the fish oil-treated group exhibited a substantial increase in Bax (p<0.05) immunolabelling and a reduction of Bcl-2 immunopositivity (p<0.05), and increased TUNEL-positive apoptotic cells (p<0.05); however, corn oil treatment did not show these beneficial effects toward mammary preneoplasia. We conclude that fish oil has the potential to play a significant role in limiting mammary tumourigenesis in vivo.
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Authors | Sangita Manna, Tridib Chakraborty, Balaram Ghosh, Mary Chatterjee, Amalendu Panda, Sunil Srivastava, Ajay Rana, Malay Chatterjee |
Journal | Prostaglandins, leukotrienes, and essential fatty acids
(Prostaglandins Leukot Essent Fatty Acids)
2008 Jul-Aug
Vol. 79
Issue 1-2
Pg. 5-14
ISSN: 0952-3278 [Print] Scotland |
PMID | 18614344
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anticarcinogenic Agents
- Bax protein, rat
- Carcinogens
- Drug Combinations
- Maxepa
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
- Docosahexaenoic Acids
- 9,10-Dimethyl-1,2-benzanthracene
- Corn Oil
- Eicosapentaenoic Acid
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Topics |
- 9,10-Dimethyl-1,2-benzanthracene
- Administration, Oral
- Animals
- Anticarcinogenic Agents
(administration & dosage)
- Apoptosis
(drug effects)
- Carcinogens
- Corn Oil
(administration & dosage)
- Disease Models, Animal
- Docosahexaenoic Acids
(administration & dosage)
- Drug Administration Schedule
- Drug Combinations
- Eicosapentaenoic Acid
(administration & dosage)
- Female
- Immunohistochemistry
- Injections, Intravenous
- Mammary Neoplasms, Experimental
(chemically induced, prevention & control)
- Proto-Oncogene Proteins c-bcl-2
(drug effects, metabolism)
- Rats
- Rats, Sprague-Dawley
- bcl-2-Associated X Protein
(drug effects, metabolism)
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