DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans.

Abstract

DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing alpha-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.

• Authors: Sheng-Fan Wang, Jason C Huang, Yuan-Ming Lee, Shih-Jen Liu, Yu-Jiun Chan, Yat-Pang Chau, Pele Chong, Yi-Ming Arthur Chen
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 373 Issue 4 Pg. 561-6 (Sep 05 2008) ISSN: 1090-2104 [Electronic] United States
• PMID: 18593570 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Cell Adhesion Molecules
• DC-specific ICAM-3 grabbing nonintegrin
• Hemagglutinin Glycoproteins, Influenza Virus
• Lectins, C-Type
• Receptors, Cell Surface
• Viral Proteins
• hemagglutinin, avian influenza A virus
• sialic acid receptor
• NA protein, influenza A virus
• Neuraminidase

Topics

• Animals
• Antibodies, Monoclonal
• Cell Adhesion Molecules (immunology)
• Cell Line
• Dendritic Cells (immunology, virology)
• Dogs
• Hemagglutination, Viral (immunology)
• Hemagglutinin Glycoproteins, Influenza Virus (chemistry, immunology)
• Humans
• Influenza A Virus, H5N1 Subtype (genetics, immunology)
• Influenza in Birds (immunology, transmission)
• Influenza, Human (immunology, transmission)
• Lectins, C-Type (immunology)
• Neuraminidase (immunology)
• Poultry (immunology, virology)
• Protein Conformation
• Receptors, Cell Surface (immunology)
• Viral Proteins (immunology)
• Virion (genetics, immunology)