Significance of Bcl-xL in human colon carcinoma.

Abstract

AIM:

To investigate the clinical significance of Bcl-xL gene in the pathogenesis of human colon carcinoma.

METHODS:

Fifty-six pair tissue samples from patients with colon cancer were collected, and protein level of the Bcl-xL gene was measured by immunohistochemistry method. The correlation of Bcl-xL expression with clinical index was evaluated. After human colon cancer cell line HT29 was transfected with Bcl-xL small interfering RNA (siRNA), the anchorage-independent growth of cancer cells was detected by colony formation in soft agar and invasion ability of cancer cells was determined by a transwell model.

RESULTS:

The Bcl-xL expression was higher in cancerous tissue samples than in normal tissue samples (38.78 +/-11.36 vs 0.89 +/- 0.35, P < 0.001), and was associated with the pathological grade, lymph node metastasis and Duke's stage of colorectal carcinoma. Transfection with Bcl-xL siRNA inhibited the colony formation and invasion ability of human colon cancer cell line HT29 in vitro.

CONCLUSION:

Bcl-xL gene plays an important role in carcinogenesis of human colorectal carcinoma and is associated with malignant biological behaviors of human colorectal carcinoma.

• Authors: You-Li Zhang, Li-Qun Pang, Ying Wu, Xiao-Yan Wang, Chong-Qiang Wang, Yu Fan
• Journal: World journal of gastroenterology (World J Gastroenterol) Vol. 14 Issue 19 Pg. 3069-73 (May 21 2008) ISSN: 1007-9327 [Print] United States
• PMID: 18494061 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• BCL2L1 protein, human
• RNA, Small Interfering
• bcl-X Protein

Topics

• Carcinoma (genetics, metabolism, pathology)
• Cell Movement
• Cell Proliferation
• Colonic Neoplasms (genetics, metabolism, pathology)
• Female
• HT29 Cells
• Humans
• Immunohistochemistry
• Lymphatic Metastasis
• Male
• Middle Aged
• Neoplasm Invasiveness
• Neoplasm Staging
• RNA Interference
• RNA, Small Interfering (metabolism)
• Time Factors
• Transfection
• bcl-X Protein (genetics, metabolism)