Local delivery of 17-beta-estradiol modulates collagen content in coronary porcine arteries after PTCA and stent implantation.

Abstract

BACKGROUND:

Percutaneous transluminal coronary angioplasty (PTCA) and stent implantation are associated with intimal hyperplasia and extracellular matrix (ECM) accumulation, resulting in restenosis. We showed that local delivery of 17-beta-estradiol (17betaE) reduced restenosis following PTCA and stent implantation by 47 and 23%, respectively. Because estrogens decreased type I and type III collagen synthesis in vitro, we hypothesized that local delivery of 17betaE may influence intimal hyperplasia formation by modulating ECM expression.

METHODS:

Porcine coronary arteries underwent PTCA or stenting and were randomly assigned to 17betaE or placebo. After 28 days, animals were sacrificed for histology and collagen type I and III content analysis.

RESULTS:

Both collagen subtypes increased in the media by 1.7 to 2.6-fold after PTCA and by 15.7 to 16.1-fold after stenting, as compared to PTCA segments. In the neointima, the ratio of collagen type III to type I was 2.7 in stented arteries and only 0.3 in PTCA arteries. In the neointima of 17betaE-treated animals, collagen type I (but not type III) content upregulation was limited by 53% after PTCA and by 74% after stenting.

CONCLUSION:

Local delivery of 17betaE reduces restenosis, in part by decreasing the density of collagen type I in the neointima in PTCA and stented arteries.

• Authors: Pedro Geraldes, Pascale Geoffroy, Isabelle Cloutier, Martin G Sirois, Jean-François Tanguay
• Journal: Journal of vascular research (J Vasc Res) Vol. 45 Issue 6 Pg. 503-11 ( 2008) ISSN: 1423-0135 [Electronic] Switzerland
• PMID: 18451633 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2008 S. Karger AG, Basel.

Chemical References

• Collagen Type I
• Collagen Type III
• Estradiol

Topics

• Angioplasty, Balloon, Coronary (adverse effects, instrumentation)
• Animals
• Collagen Type I (metabolism)
• Collagen Type III (metabolism)
• Coronary Restenosis (etiology, metabolism, pathology, prevention & control)
• Coronary Vessels (drug effects, metabolism, pathology)
• Disease Models, Animal
• Estradiol (administration & dosage)
• Humans
• Hyperplasia
• Male
• Stents
• Swine