Post-transcriptional regulation of breast cancer resistance protein after intestinal ischemia-reperfusion.

Abstract

Breast cancer resistance protein (BCRP), the product of the ABCG2 gene, is a recently identified ATP binding cassette half-transporter. BCRP is expressed in a variety of tumor cells and many normal human tissues. In the small intestine, BCRP can limit the influx and facilitate the efflux to prevent intracellular accumulation of BCRP substrates. Ischemia-reperfusion (I/R) induces the release of reactive oxygen species, and organs are severely damaged by I/R. It has been shown that the expression of transporters was altered in the organ after I/R. The present study was undertaken to clarify the expression of BCRP after intestinal I/R. We showed that the expression level of Bcrp was significantly decreased at 1 h after I/R. Bcrp mRNA level was not altered at 1 h after I/R. These results suggest that Bcrp expression was regulated by a post-transcriptional regulation mechanism after intestinal I/R. Bcrp mRNA level was increased at 24 h after I/R, and the expression level of Bcrp protein was of the same level or slightly increased compared with sham operated-rats. Bcrp was slightly located at the intestinal membrane at 24 h after intestinal I/R. These results suggested that Bcrp was not translocated to the intestinal membrane after intestinal I/R. There is little information on post-transcriptional regulation compared with information on transcriptional regulation. In this study, it was shown that Bcrp expression is regulated by post-transcriptional regulation after intestinal I/R. These results of this study may provide important information for further studies aimed at revealing the biological function of Bcrp.

• Authors: Jiro Ogura, Masaki Kobayashi, Shirou Itagaki, Takeshi Hirano, Ken Iseki
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 31 Issue 5 Pg. 1032-5 (May 2008) ISSN: 0918-6158 [Print] Japan
• PMID: 18451542 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• ATP Binding Cassette Transporter, Subfamily G, Member 2
• ATP-Binding Cassette Transporters
• Abcg2 protein, rat

Topics

• ATP Binding Cassette Transporter, Subfamily G, Member 2
• ATP-Binding Cassette Transporters (biosynthesis)
• Animals
• Blotting, Western
• Drug Resistance, Neoplasm
• Ileum (blood supply, metabolism)
• Immunohistochemistry
• Intestines (blood supply, physiopathology)
• Male
• Protein Processing, Post-Translational (physiology)
• Rats
• Rats, Wistar
• Regional Blood Flow (physiology)
• Reperfusion Injury (metabolism, physiopathology)
• Reverse Transcriptase Polymerase Chain Reaction