Abstract |
The polyphenol trans-resveratrol (t-RES) is present as t-RES-3-O-beta-D-glycoside, termed piceid, in several plant-derived foods. Although data on the metabolism and on in vivo effects of t-RES have been reported, quantitative data on the metabolites formed after dietary intake of t-RES or piceid are still lacking. In this study, 85.5 mg of piceid per 70 kg of body weight (bw) were administered to healthy volunteers in a bolus dose. t-RES metabolites formed in plasma and urine were identified and quantified by LC-MS/MS, NMR, and HPLC-DAD analysis using chemically synthesized t-RES conjugate standards. In addition, the amount of t-RES metabolites bound noncovalently to plasma proteins was determined for the first time in humans. The metabolites identified and quantified were t-RES-3-sulfate, t-RES-3,4'-disulfate, t-RES-3,5-disulfate, t-RES-3-glucuronide and t-RES-4'-glucuronide, with t-RES- sulfates being the dominant conjugates in plasma and urine. Besides these metabolites, two novel t-RES-C/O-conjugated diglucuronides have been identified and quantified in plasma and urine. Moreover, it could be shown that up to 50% of the plasma t-RES-3-sulfate, t-RES-disulfates, and the novel t-RES-C/O-diglucuronides were bound to proteins. Total recovery of the dietary administered piceid in urine ranged between 13.6 and 35.7%.
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Authors | Alexander Burkon, Veronika Somoza |
Journal | Molecular nutrition & food research
(Mol Nutr Food Res)
Vol. 52
Issue 5
Pg. 549-57
(May 2008)
ISSN: 1613-4133 [Electronic] Germany |
PMID | 18435437
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antineoplastic Agents, Phytogenic
- Blood Proteins
- Glucuronides
- Stilbenes
- Resveratrol
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Topics |
- Adult
- Antineoplastic Agents, Phytogenic
(blood, metabolism, urine)
- Blood Proteins
(metabolism)
- Blotting, Western
- Chromatography, High Pressure Liquid
- Glucuronides
(blood, metabolism, urine)
- Humans
- Magnetic Resonance Spectroscopy
- Male
- Models, Molecular
- Molecular Conformation
- Protein Binding
- Reference Values
- Resveratrol
- Stilbenes
(blood, chemistry, pharmacokinetics, urine)
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