Identification of antiviral mimetic peptides with interferon alpha-2b-like activity from a random peptide library using a novel functional biopanning method.

Abstract

AIM:

To screen for interferon (IFN) alpha-2b mimetic peptides with antiviral activity.

METHODS:

Selecting IFN receptor-binding peptides from a phage-display heptapeptide library using a novel functional biopanning method. This method was developed to identify peptides with activity against vesicular stomatitis virus (VSV) inducing cytopathic effects on WISH cells.

RESULTS:

Sixteen positive clones were obtained after 3 rounds of functional selection. Ten clones were picked from these positive clones according to the results of phage ELISA and were sequenced. The amino acid sequences homologous to IFN alpha-2b were defined by residues AB loop 31-37, BC loop 68-74, C helix 93-99, CD loop 106-112, D helix 115-121, DE loop 132-138, and E helix 143-161. Two of the peptides, designated clones T3 and T9, aligned with the IFNAR2-binding domains (AB loop and E helix), were synthesized and designated as IR-7 and KP-7, respectively. Both KP-7 and IR-7 were found to compete with GFP/IFN alpha-2b for receptor binding and mimicked the antiviral activity of IFN alpha -2b cooperatively.

CONCLUSION:

Two IFN alpha-2b mimetic peptides with antiviral activity were derived from a phage-display heptapeptide library using a novel functional selection method.

• Authors: Qi Zhang, Gang Bai, Jia-Qi Chen, Wang Tian, Yu Cao, Peng-Wei Pan, Chao Wang
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 29 Issue 5 Pg. 634-40 (May 2008) ISSN: 1745-7254 [Electronic] United States
• PMID: 18430373 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antiviral Agents
• Formazans
• Interferon alpha-2
• Interferon-alpha
• Peptide Library
• Peptides
• Recombinant Proteins
• Tetrazolium Salts
• MTT formazan

Topics

• Amino Acid Sequence
• Antiviral Agents (immunology)
• Cell Line
• Formazans (metabolism)
• Humans
• Interferon alpha-2
• Interferon-alpha (immunology, metabolism)
• Molecular Mimicry (drug effects)
• Molecular Sequence Data
• Peptide Library
• Peptides (chemistry, metabolism, pharmacology)
• Recombinant Proteins
• Tetrazolium Salts (metabolism)
• Vesicular stomatitis Indiana virus (immunology, metabolism)