Clinicopathological significance of the gene expression of matrix metalloproteinases and reversion-inducing cysteine-rich protein with Kazal motifs in patients with colorectal cancer: MMP-2 gene expression is a useful predictor of liver metastasis from colorectal cancer.

Abstract

Matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) and membrane-type matrix metalloproteinase 1 (MT1-MMP) are involved in colorectal cancer invasion and metastasis. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) inhibits MMP-2, MMP-9 and MT1-MMP. We examined the clinicopathological significance of the relative expression of these genes in patients with colorectal cancer, especially with regard to metastasis. We studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 205 patients with untreated colorectal carcinoma. MMP-2, MMP-9, MT1-MMP, RECK and beta-actin mRNA of cancer tissue and adjacent normal mucosa were measured by quantitative real-time reverse-transcriptase polymerase chain reaction. MT1-MMP gene expression was higher in cancer tissue than in adjacent normal mucosa. In contrast, MMP-2, MMP-9 and RECK gene expression levels were lower in cancer tissue than in adjacent normal mucosa. As for the relationship between the gene expression and clinicopathological factors, MMP-2 expression correlated with the depth of invasion, venous invasion and liver metastasis; MMP-9 and RECK expression correlated with venous invasion. There were positive correlations among the gene expression levels of MMP-2, MMP-9 and RECK. MMP-2 gene expression was considered a useful predictor of liver metastasis from colorectal cancer.

• Authors: Takashi Oshima, Chikara Kunisaki, Kazue Yoshihara, Roppei Yamada, Naoto Yamamoto, Tsutomu Sato, Hirochika Makino, Shigeru Yamagishi, Yasuhiko Nagano, Shoich Fujii, Manabu Shiozawa, Makoto Akaike, Nobuyuki Wada, Yasushi Rino, Munetaka Masuda, Katsuaki Tanaka, Toshio Imada
• Journal: Oncology reports (Oncol Rep) Vol. 19 Issue 5 Pg. 1285-91 (May 2008) ISSN: 1021-335X [Print] Greece
• PMID: 18425389 (Publication Type: Journal Article)

Chemical References

• GPI-Linked Proteins
• Ligands
• Membrane Glycoproteins
• RECK protein, human
• Matrix Metalloproteinases
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Aged
• Amino Acid Motifs
• Colorectal Neoplasms (enzymology, metabolism, pathology)
• Female
• GPI-Linked Proteins
• Gene Expression Regulation, Neoplastic
• Humans
• Ligands
• Liver Neoplasms (metabolism, secondary)
• Male
• Matrix Metalloproteinase 2 (biosynthesis)
• Matrix Metalloproteinase 9 (biosynthesis)
• Matrix Metalloproteinases (biosynthesis)
• Membrane Glycoproteins (biosynthesis)
• Middle Aged
• Neoplasm Metastasis