Abstract | OBJECTIVE: METHODS: Rats were randomized to six groups: DMBA + glutamine, DMBA + Freamine, DMBA + water, oil + glutamine, oil + Freamine, or oil + water. At age 50 d, rats were gavaged with DMBA or sesame oil. Rats also received glutamine, Freamine, or water by gavage from 1 wk before DMBA administration until sacrifice at 1, 2, 4, or 11 wk after DMBA. Tumor appearance, blood, gut mucosa and breast glutamine and/or glutathione concentrations, and NK cell cytotoxicity were measured. The gut extractions, defined as the difference of concentrations across the gut, were calculated. RESULTS: Oral glutamine reduced the tumorigenesis of DMBA by 50% in this model. DMBA altered the difference of glutathione concentrations across the gut; however, oral glutamine maintained the normal gut glutathione release. NK cell activities were lower in DMBA groups at early (week 1) and late (week 11) time points, but oral glutamine recovered the NK cell activity only at week 11. In addition, blood, gut, and breast glutathione concentrations were enhanced by glutamine supplementation. CONCLUSION: These results indicate that one of the mechanisms of dietary glutamine anticancer action is through upregulating gut glutathione metabolism.
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Authors | Yihong Kaufmann, Paul Spring, V Suzanne Klimberg |
Journal | Nutrition (Burbank, Los Angeles County, Calif.)
(Nutrition)
Vol. 24
Issue 5
Pg. 462-9
(May 2008)
ISSN: 0899-9007 [Print] United States |
PMID | 18313901
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Glutamine
- 9,10-Dimethyl-1,2-benzanthracene
- Glutathione
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Topics |
- 9,10-Dimethyl-1,2-benzanthracene
- Administration, Oral
- Animals
- Disease Models, Animal
- Female
- Glutamine
(administration & dosage)
- Glutathione
(metabolism)
- Killer Cells, Natural
(drug effects, metabolism)
- Mammary Neoplasms, Experimental
(chemically induced, prevention & control)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Time Factors
- Up-Regulation
(drug effects)
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