Cross-reactive IgM responses in patients with dengue or Japanese encephalitis.

Abstract

BACKGROUND:

Dengue and Japanese encephalitis viruses co-circulate in Thailand. IgM-capture enzyme-linked immunosorbent assay (ELISA) has been widely used for confirmation of dengue and Japanese encephalitis (JE).

OBJECTIVES:

To examine the cross-reactivity in IgM responses to dengue and JE viruses in serum and CSF samples from dengue and JE patients.

STUDY DESIGN:

Two hundred and fifty-eight serum samples from 177 confirmed dengue patients, and 99 serum samples and 37 cerebrospinal fluid (CSF) samples from confirmed JE patients were analyzed.

RESULTS:

Nine percent of serum samples from dengue patients were positive for anti-JE IgM. Thirteen percent of serum samples and 11% of CSF samples from JE patients were positive for anti-dengue IgM. Levels of cross-reactive IgM were lower than those of specific IgM in all the dengue and JE patients.

CONCLUSIONS:

Only specific IgM is detected in about 90% of dengue and JE patients, but cross-reactive IgM is also detected in the remainder. The presence of cross-reactive IgM responses should to be considered in the serodiagnosis of dengue and JE, especially in areas where dengue and JE viruses co-circulate.

• Authors: Atchareeya A-Nuegoonpipat, Nirandorn Panthuyosri, Surapee Anantapreecha, Sumalee Chanama, Areerat Sa-Ngasang, Pathom Sawanpanyalert, Ichiro Kurane
• Journal: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology (J Clin Virol) Vol. 42 Issue 1 Pg. 75-7 (May 2008) ISSN: 1386-6532 [Print] Netherlands
• PMID: 18304870 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Viral
• Immunoglobulin M

Topics

• Antibodies, Viral (blood, cerebrospinal fluid)
• Cross Reactions
• Dengue (immunology)
• Encephalitis, Japanese (immunology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Humans
• Immunoglobulin M (blood, cerebrospinal fluid)
• Thailand