Abstract |
A growing body of evidence has shown that professional antigen-presenting cells (APC) treated with transforming growth factor-beta ( TGF-beta) can induce a systemic antigen (Ag)-specific tolerance, similar to anterior chamber-associated immune deviation (ACAID). However, the exact mechanism for immune tolerance induced by TGF-beta-treated APC has not been elucidated. In this study, we showed that intravenous injection of ovalbumin (OVA)-pulsed APC treated with TGF-beta(2) induced a peripheral tolerance as evidenced by an impaired delayed-type hypersensitivity (DTH) response. CD4(+) T cells from mice receiving an intravenous injection of TGF-beta(2)-treated APC pulsed with OVA could adoptively transfer a specific tolerance to naïve mice. An increased frequency of FoxP3-expressing CD4(+) CD25(+) T cells was observed in mice with tolerance. CD4(+ )CD25(+) T cells from TGF-beta(2)-treated APC-injected mice produced a large amount of TGF-beta(1) and exhibited an in vitro antigen-specific suppressive activity. CD4(+) CD25(+) T cells from TGF-beta(2)-treated APC-injected mice were able to inhibit the antigen-specific DTH response significantly when adoptively transferred to naïve mice. These results indicate that FoxP3-expressing CD4(+) CD25(+) T cells might be actively involved in the development of tolerance induced by TGF-beta(2)-treated APC.
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Authors | Haining Zhang, Peizeng Yang, Hongyan Zhou, Qianli Meng, Xiangkun Huang |
Journal | Immunology
(Immunology)
Vol. 124
Issue 3
Pg. 304-14
(Jul 2008)
ISSN: 1365-2567 [Electronic] England |
PMID | 18266851
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Forkhead Transcription Factors
- Foxp3 protein, mouse
- Interleukin-2 Receptor alpha Subunit
- Tgfb2 protein, mouse
- Transforming Growth Factor beta2
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Topics |
- Adoptive Transfer
- Animals
- Antigen-Presenting Cells
(immunology, transplantation)
- Cells, Cultured
- Female
- Forkhead Transcription Factors
(metabolism)
- Immune Tolerance
(immunology)
- Interleukin-2 Receptor alpha Subunit
(analysis)
- Macrophages, Peritoneal
(immunology)
- Mice
- Mice, Inbred BALB C
- T-Lymphocytes, Regulatory
(immunology)
- Transforming Growth Factor beta2
(immunology)
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