Expression of Ki-67, p53 and p63 proteins in keratocyst odontogenic tumours: an immunohistochemical study.

Abstract

AIM:

To investigate the immunohistochemical expression of Ki-67, p53 and p63 in Keratocyst Odontogenic Tumours (KOTs) in order to contribute to the biological profile of this tumor.

METHODS:

Immunohistochemical technique was performed using the EnVision System in 37 cases of KOTs.

RESULTS:

Ki-67- and p53-immunostained cells were mainly located in the suprabasal layers. p63-positive cells were found throughout the lining cystic epithelium. No difference in the immunostaining for these proteins was observed between primary and recurrent KOTs (Ki-67: P = 0.5591; p53: P = 0.9847; p63: P = 0.9127), or between KOTs associated with Nevoid Basal Cell Carcinoma Syndrome (NBCCS) and sporadic KOTs (Ki-67: P = 0.7013; p53: P = 0.3197; p63: P = 0.2427).

CONCLUSIONS:

It is possible that biological behavior of KOTs may be related to suprabasal proliferative compartment in the cystic epithelium as observed by high levels of Ki-67, p53 and p63. In addition, p63 immunostaining may represent immaturity of keratinocytes in KOTs, and suggests that this protein may participate in the regulation of epithelial cell differentiation. Taken together, these data may favor tumorigenesis on KOTs.

• Authors: Clarissa Araújo Silva Gurgel, Eduardo Antônio Gonçalves Ramos, Roberto Almeida Azevedo, Viviane Almeida Sarmento, Ana Maria da Silva Carvalho, Jean Nunes dos Santos
• Journal: Journal of molecular histology (J Mol Histol) Vol. 39 Issue 3 Pg. 311-6 (Jun 2008) ISSN: 1567-2379 [Print] Netherlands
• PMID: 18256893 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies
• Ki-67 Antigen
• TP63 protein, human
• Trans-Activators
• Transcription Factors
• Tumor Suppressor Protein p53
• Tumor Suppressor Proteins

Topics

• Antibodies
• Cell Count
• Humans
• Immunohistochemistry
• Ki-67 Antigen (metabolism)
• Odontogenic Cysts (metabolism, pathology)
• Odontogenic Tumors (metabolism, pathology)
• Trans-Activators (metabolism)
• Transcription Factors
• Tumor Suppressor Protein p53 (metabolism)
• Tumor Suppressor Proteins (metabolism)