Abstract |
limitations in current technology for generating transgenic animals, such as the time and the expense, hampered its extensive use in recombinant protein production for therapeutic purpose. In this report, we present a simple and less expensive alternative by directly infusing a recombinant adenovirus vector carrying human lactoferrin cDNA into rabbit mammary glands. The milk serum was collected from the infected mammary gland 48 h post- infection and subjected to a 10% SDS-PAGE and Western blotting. An 80-kDa protein was visualized after viral vector infection. With this method, we obtained a high level of expressed human lactoferrin of up to 2.3 mg/ml in the milk. Taken together, the method is useful for the transient high-level expression recombinant proteins, and the approach established here is probably one of the most economical and efficient ways for large-scale production of recombinant proteins of biopharmaceutical interest.
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Authors | Zeng-Sheng Han, Qing-Wang Li, Zhi-Ying Zhang, Yong-Sheng Yu, Bo Xiao, Shu-Yun Wu, Zhong-Liang Jiang, Hong-Wei Zhao, Rui Zhao, Jian Li |
Journal | Journal of microbiology and biotechnology
(J Microbiol Biotechnol)
Vol. 18
Issue 1
Pg. 153-9
(Jan 2008)
ISSN: 1017-7825 [Print] Korea (South) |
PMID | 18239433
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
- Recombinant Proteins
- Lactoferrin
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Topics |
- Adenoviridae
(genetics, metabolism)
- Animals
- Biotechnology
(methods)
- Cell Line
- Cells, Cultured
- Epithelial Cells
- Female
- Genetic Vectors
- Humans
- Lactoferrin
(genetics, metabolism)
- Mammary Glands, Animal
(cytology, virology)
- Milk
(metabolism)
- Rabbits
- Recombinant Proteins
(genetics, metabolism)
- Transduction, Genetic
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