Abstract | PURPOSE: METHODS: The animals were divided into three groups: Group A--normal fetuses from pregnant rats that were not administered ETU; Group B--fetuses without ARM born from pregnant rats that were administered ETU and Group C--fetuses with ARM born from pregnant rats that received ETU. ETU was administered on the 11th day of pregnancy at the dose of 125 mg/kg body weight by gastric gavage. The rats had cesarean section on the 21st day of gestation. The fetuses terminal bowel tissue was analyzed by immunohistochemistry to demonstrate ganglion cells. RESULTS: Statistically significant differences were found between groups A, B and C regarding ganglion cell densities. Group A had the highest cell density, followed by Group B and the lowest density was found in Group C. CONCLUSION:
Ganglion cell densities are decreased in the terminal bowel of rats with ARM.
|
Authors | Maurício Macedo, José Luiz Martins, Karine Furtado Meyer, Iberê Cauduro Soares |
Journal | Acta cirurgica brasileira
(Acta Cir Bras)
2007 Nov-Dec
Vol. 22
Issue 6
Pg. 441-5
ISSN: 0102-8650 [Print] Brazil |
PMID | 18235931
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- S100 Proteins
- Ethylenethiourea
|
Topics |
- Anal Canal
(abnormalities, innervation, pathology)
- Animals
- Cell Count
- Disease Models, Animal
- Enteric Nervous System
(drug effects, pathology)
- Ethylenethiourea
- Female
- Ganglia
(cytology)
- Immunohistochemistry
- Pregnancy
- Rats
- Rats, Wistar
- Rectum
(abnormalities, innervation, pathology)
- S100 Proteins
(analysis)
|