AMP N1-oxide potentiates astrogenesis by cultured neural stem/progenitor cells through STAT3 activation.

Abstract

We earlier identified adenosine monophosphate (AMP) N1-oxide as a unique compound of royal jelly (RJ) that induces neurite outgrowth from cultured rat pheochromocytoma PC12 cells. In the present study, the effects of AMP N1-oxide on the proliferation and/or differentiation of cultured neural stem/progenitor cells (NSCs) were examined. As for cell proliferation, low micromolar concentrations of AMP N1-oxide or its parent compound, AMP, similarly enhanced the NSC proliferation-inducing activity of basic fibroblast growth factor (FGF-2), although neither compound tested alone affected cell proliferation. Conversely, high concentrations of AMP N1-oxide (over 20 microM) markedly suppressed cell growth even in the presence of FGF-2. However, this suppression was not observed with AMP. As for cell differentiation, AMP N1-oxide, but not AMP, increased the generation of astrocytes in a dose-dependent manner when the cells were cultured in medium lacking FGF-2. The generation of neurons or oligodendrocytes was not influenced by AMP N1-oxide. Furthermore, AMP N1-oxide increased the phosphorylation of STAT3 (signal transducer and activator of transcription 3), a transcription factor that mediates the expression of astrocytespecific genes. These results suggest that AMP N1-oxide is one of the components that facilitates astrogenesis by NSCs through activation of STAT3.

• Authors: Noriko Hattori, Hiroshi Nomoto, Hidefumi Fukumitsu, Satoshi Mishima, Shoei Furukawa
• Journal: Biomedical research (Tokyo, Japan) (Biomed Res) Vol. 28 Issue 6 Pg. 295-9 (Dec 2007) ISSN: 1880-313X [Electronic] Japan
• PMID: 18202519 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AMP N1-oxide
• STAT3 Transcription Factor
• Stat3 protein, rat
• Adenosine Monophosphate

Topics

• Adenosine Monophosphate (analogs & derivatives, pharmacology, physiology)
• Animals
• Astrocytes (cytology, drug effects, metabolism)
• Cell Differentiation
• Cell Proliferation
• Cells, Cultured
• Neurites (drug effects, metabolism)
• Neurons (cytology, drug effects, metabolism)
• Rats
• STAT3 Transcription Factor (metabolism)
• Signal Transduction
• Stem Cells (cytology, drug effects, metabolism)