RNA from brush oral cytology to measure squamous cell carcinoma gene expression.

Abstract

BACKGROUND:

RNA expression analysis of oral keratinocytes can be used to detect early stages of disease such as oral cancer or to monitor on-going treatment responses of the same or other oral diseases. A limitation is the inability to obtain high quality RNA from oral tissue without using biopsies. While oral cytology cell samples can be obtained from patients in a minimally invasive manner they have not been validated for quantitative analysis of RNA expression.

METHODS:

As a starting point in the analysis of tumor markers in oral squamous cell carcinoma (OSCC), we examined RNA in brush cytology samples from hamsters treated with dibenzo[a,l]pyrene to induce oral carcinoma. Three separate samples from each animal were assessed for expression of candidate marker genes and control genes measured with real-time RT-PCR.

RESULTS:

Brush oral cytology samples from normal mucosa were shown to consist almost exclusively of epithelial cells. Remarkably, ss-2 microglobulin and cytochrome p450, 1B1 (CYP1B1) RNA showed potential utility as markers of OSCC in samples obtained in this rapid and non-surgical manner.

CONCLUSION:

Brush oral cytology may prove useful as a source of RNA for gene expression analysis during the progression of diseases of the oral epithelium such as OSCC.

• Authors: Joel L Schwartz, Suchismita Panda, Craig Beam, Laura E Bach, Guy R Adami
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 37 Issue 2 Pg. 70-7 (Feb 2008) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 18197850 (Publication Type: Evaluation Study, Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Benzopyrenes
• Neoplasm Proteins
• RNA, Neoplasm
• dibenzo(a,i)pyrene

Topics

• Animals
• Benzopyrenes
• Biopsy (methods, statistics & numerical data)
• Carcinoma, Squamous Cell (chemically induced, chemistry, genetics, pathology)
• Cricetinae
• Feasibility Studies
• Gene Expression
• Keratinocytes (pathology)
• Mesocricetus
• Mouth Neoplasms (chemically induced, chemistry, genetics, pathology)
• Neoplasm Proteins (analysis, genetics)
• RNA, Neoplasm (analysis)
• Reproducibility of Results
• Reverse Transcriptase Polymerase Chain Reaction