[Spontaneous response and mechanism to ischemia in diabetic mouse].

Abstract

OBJECTIVE:

To investigate the spontaneous response and its mechanism of type 2 diabetes mouse to surgical induced hind limb ischemia.

METHODS:

Forty mice were randomly divided into two groups, one was fed with normal chow, and another was fed with high fat diet. Twelve weeks later, mice were surgical induced to hind limb ischemia. Blood flow was monitored. Four weeks later, mice were sacrificed and muscle tissues were harvested for histological detection, Western Blot, ELISA and RT-PCR examination respectively.

RESULTS:

Spontaneous restoration of blood flow was significantly slow down in type 2 diabetic mice with lower level of vascular density in ischemic muscles. VEGF signaling pathway was impaired although the VEGF levels were increased in diabetes.

CONCLUSIONS:

The spontaneous response to ischemia was attenuated with an abnormal reaction of VEGF signaling pathway in the setting of type 2 diabetes mellitus.

• Authors: Yong-jun Li, Qing Lin, Chang-wei Liu, Heng Guan, Xia-lin Liu
• Journal: Zhonghua wai ke za zhi [Chinese journal of surgery] (Zhonghua Wai Ke Za Zhi) Vol. 45 Issue 17 Pg. 1206-9 (Sep 01 2007) ISSN: 0529-5815 [Print] China
• PMID: 18067719 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Vascular Endothelial Growth Factor A
• Receptors, Vascular Endothelial Growth Factor

Topics

• Animals
• Blotting, Western
• Diabetes Mellitus, Type 2 (complications, physiopathology)
• Disease Models, Animal
• Enzyme-Linked Immunosorbent Assay
• Hindlimb (blood supply, metabolism)
• Ischemia (etiology, physiopathology)
• Male
• Mice
• Mice, Inbred C57BL
• Neovascularization, Pathologic (genetics, metabolism, physiopathology)
• Random Allocation
• Receptors, Vascular Endothelial Growth Factor (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Signal Transduction
• Vascular Endothelial Growth Factor A (metabolism)