5-fluoro-2'-deoxyuridine (
FUdR) inhibits
thymidylate synthase. We have been investigated the molecular mechanisms of cell death in mouse mammary
tumor FM3A cells, F28-7 strain and its mutant F28-7-A strain, after treated with
FUdR. Previously, we have been reported that F28-7 strain induced DNA cleavage into chromosomal sized fragments and subsequently develop
necrosis, but F28-7-A strain induced DNA cleavage into oligonucleosomal sized fragments and subsequently develop apoptosis after treated with
FUdR. To understand the molecular mechanisms of regulate of two differential cell death
necrosis and apoptosis, we identify cell death regulator by using
proteome and transcriptome analysis. When compared with the
proteome of F28-7 and F28-7-A strain after treated with
FUdR, it was found that 5
proteins were up-regulated and 11
proteins were down-regulated in F28-7-A strain. Furthermore, transcriptome analysis shows that 94 genes were up-regulated and 164 genes were downregulated in F28-7-A strain. Identified
proteins and genes were involved in various cellular processes such as cell cycle regulation, apoptosis, proliferation, and differentiation. Our results suggested that numerous features indicated the coordinated regulation of molecular networks from various aspects of
necrosis or apoptosis at the
proteome and transcriptome levels.