[Characterization and biological significance of peripheral blood dendritic cell subsets in patients with severe burn].

Abstract

OBJECTIVE:

To investigate the characteristics of dendritic cells (DCs) in patients with severe burn.

METHODS:

Peripheral blood samples were obtained from 12 patients with severe burn 1, 2, 3, and 4 weeks after burn, and from 10 healthy donors as controls. Mononuclear cells were isolated, and GM-CSF 1000 and IL-4 were used to induce the transformation of DCs. Mature DCs were harvested and underwent flow cytometry to detect the expression of HLA-DR, CD80, CD83, CD86, CD14, and CD11c.

RESULT:

1, 2, 3, and 4 weeks after burn, the expression levels of HLA-DR were 62.1% +/- 8.4%, 65.0% +/- 6.2%, 68.4% +/- 5.7%, and 75.4% +/- 8.4% respectively; the expression levels of CD80 were 12.9% +/- 3.7%, 14.7% +/- 2.5%, 16.1% +/- 4.2%, and 16.2% +/- 4.8%; the expression levels of CD83 were 15.1% +/- 4.2%, 15.1% +/- 4.0%, 22.2% +/- 7.7%, and 21.3% +/- 7.0% respectively; the expression levels of CD86 were 69.2% +/- 7.1%, 70.5% +/- 5.4%, 75.1% +/- 6.1%, and 79.6% +/- 6.4% respectively, all significantly lower than that of the control group (84.3% +/- 8.2%, all P < 0.001). 1, 2, 3, and 4 weeks after burn, the expression levels of CD14 in the DCs of the severe burn patients were 12.7% +/- 1.9%, 12.0% +/- 1.5%, 11.3% +/- 1.3%, and 9.3% +/- 1.7% respectively, all significantly higher than that of the control group (7.3% +/- 1.5%, P < 0.001). 1, 2, 3, and 4 weeks after burn, the expression levels of CD11c in the DCs of the severe burn patients were 86.8% +/- 6.1%, 89.5% +/- 5.1%, 91.3% +/- 2.8%, and 89.4% +/- 4.0% respectively, a little lower than that of the control group, however, without significant difference (92.6% +/- 3.8%, P > 0.05).

CONCLUSION:

The functions of DCs in the patients with severe burn are declined, which may partly contribute to infection after burn.

• Authors: Pu-Zhu Zhang, Feng-Jun Qin, Chun-Xu Ma, Hong Su, Dong-Ning Yu, Chi Li
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 87 Issue 32 Pg. 2275-7 (Aug 28 2007) ISSN: 0376-2491 [Print] China
• PMID: 18001551 (Publication Type: Journal Article)

Chemical References

• Antigens, CD
• Antigens, CD34
• B7-1 Antigen
• B7-2 Antigen
• CD11c Antigen
• HLA-DR Antigens
• Immunoglobulins
• Lipopolysaccharide Receptors
• Membrane Glycoproteins

Topics

• Adolescent
• Adult
• Antigens, CD (biosynthesis)
• Antigens, CD34 (biosynthesis)
• B7-1 Antigen (biosynthesis)
• B7-2 Antigen (biosynthesis)
• Burns (blood, immunology, pathology)
• CD11c Antigen (biosynthesis)
• Cells, Cultured
• Dendritic Cells (metabolism)
• Female
• Flow Cytometry
• HLA-DR Antigens (biosynthesis)
• Humans
• Immunoglobulins (biosynthesis)
• Lipopolysaccharide Receptors (biosynthesis)
• Male
• Membrane Glycoproteins (biosynthesis)
• Middle Aged
• CD83 Antigen